ZHAO Hui, ZHANG Yuefan, LI Tiejun. Protective effect and mechanism of sodium dichloroacetate on BV2 cells damaged by OGD[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 146-150. doi: 10.3969/j.issn.1006-0111.2019.02.009
| Citation:
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ZHAO Hui, ZHANG Yuefan, LI Tiejun. Protective effect and mechanism of sodium dichloroacetate on BV2 cells damaged by OGD[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 146-150. doi: 10.3969/j.issn.1006-0111.2019.02.009
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Protective effect and mechanism of sodium dichloroacetate on BV2 cells damaged by OGD
- 1.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
- 2.
Department of Pharmacology, School of Pharmacy, Naval Medical University, Shanghai 200433, China
- 3.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, Chian
- Received Date: 2018-10-20
- Rev Recd Date:
2018-12-27
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Abstract
Objective To study the protective effect and mechanism of dichloroacetate (DCA) on BV2 microglia in oxygen glucose deprivation (OGD) injury model. Methods BV2 cells were divided into 3 groups:CON group,OGD group and DCA intervention group.The injury model was established by OGD 4 h.CCK-8 and flow cytometry was used to detect apoptosis and expression of ROS and NO in cells.Western blot was used to detect the expression of proteins related to NF-κB pathway. Results CCK-8 and flow cytometry showed that the apoptosis of BV2 cells induced by OGD and the expression of ROS and NO in OGD cells were decreased significantly by DCA (P<0.05).Western blot analysis showed that the expression of I-κB,JNK and NF-κB protein in BV2 cells mediated by OGD injury were influenced significantly by DCA (P<0.05). Conclusion DCA had a protective effect on OGD-injured BV2 cells through anti-oxidation and anti-apoptosis.
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