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Volume 37 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(2): 121-125

LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
Citation: LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
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Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats

doi: 10.3969/j.issn.1006-0111.2019.02.005
  • 1.

    Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China

  • 2.

    Shanghai Jiaotong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China

  • Received Date: 2018-06-11
  • Rev Recd Date: 2018-10-15
  • Objective To explore the impact of chronic unpredicted mild stress (CUMS)induced depression on mRNA and protein expression of liver organic anion transporting polypeptides (Oatps) in rats. Methods Twenty-four male SD rats were divided into depression group and control group.Rats in depression group were exposed CUMS for 8 weeks consecutively.Weight,open-field test,sucrose preference,plasma concentration of noradrenaline and corticosterone were assayed before and after model establishment to evaluate the depression.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were measured by RT-qPCR and Western blotting,respectively. Results In CUMS induced depression group,weight,sucrose consumption,vertical score and horizontal score were decreased while levels of corticosterone and norepinephrine were increased compared with control group.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were reduced compared with control group. Conclusion The mRNA and protein expression of liver Oatps in rats were regulated down by CUMS induced depression.
  • [1] KOVACSICS D,PATIK I,ÖZVEGY-LACZKA C.The role of organic anion transporting polypeptides in drug absorption,distribution,excretion and drug-drug interactions[J].Expert Opin Drug Metab Toxicol,2017,13(4):409-424.
    [2] HAGENBUCH B,MEIER P J.Organic anion transporting polypeptides of the OATP/SLC21 family:phylogenetic classification as OATP/SLCO superfamily,new nomenclature and molecular/functional properties[J].Pflugers Arch,2004,447(5):653-665.
    [3] WEI H,ZHOU T,TAN B,et al. Impact of chronic unpredicted mild stress-induced depression on repaglinide fate via glucocorticoid signaling pathway[J].Oncotarget,2017,8(27):44351-44365.
    [4] XIA Z,WEI H,DUAN J,et al. Chronic unpredicted mild stress-induced depression alter saxagliptin pharmacokinetics and CYP450 activity in GK rats[J].PeerJ,2016,4:e1611.
    [5] ZENG Y,XIE X,DUAN J,et al. Perturbation of mitiglinide metabolism by chronic unpredicted mild stress in rats[J].Sci Rep,2014,4:3794.
    [6] YANG L,ZHAO Y,WANG Y,et al. The Effects of psychological stress on depression[J].Curr Neuropharmacol,2015,13(4):494-504.
    [7] WU G F,REN S,TANG R Y,et al. Antidepressant effect of taurine in chronic unpredictable mild stress-induced depressive rats[J].Sci Rep,2017,7(1):4989.
    [8] WANG B,WANG Y,WU Q,et al. Effects of α2A Adrenoceptors on Norepinephrine Secretion from the Locus Coeruleus during Chronic Stress-Induced Depression[J].Front Neurosci,2017,11:243.
    [9] GONG I Y,KIM R B.Impact of genetic variation in OATP transporters to drug disposition and response[J].Drug Metab Pharmacokinet,2013,28(1):4-18.
    [10] HAGENBUCH B,STIEGER B.The SLCO (former SLC21) superfamily of transporters[J].Mol Aspects Med,2013,34(2-3):396-412.
    [11] HARTMANN G,CHEUNG A K,PIQUETTE-MILLER M.Inflammatory cytokines,but not bile acids,regulate expression of murine hepatic anion transporters in endotoxemia[J].J Pharmacol Exp Ther,2002,303(1):273-281.
    [12] HAYLEY S.Toward an anti-inflammatory strategy for depression[J].Front Behav Neurosci,2011,5:19.
    [13] YOU Z,LUO C,ZHANG W,et al. Pro-and anti-inflammatory cytokines expression in rat's brain and spleen exposed to chronic mild stress:involvement in depression[J].Behav Brain Res,2011,225(1):135-141.
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Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats

doi: 10.3969/j.issn.1006-0111.2019.02.005
  • 1. Shanghai University of Medicine & Health Sciences Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China
  • 2. Shanghai Jiaotong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, China
  • Received Date: 2018-06-11
  • Rev Recd Date: 2018-10-15

Abstract: Objective To explore the impact of chronic unpredicted mild stress (CUMS)induced depression on mRNA and protein expression of liver organic anion transporting polypeptides (Oatps) in rats. Methods Twenty-four male SD rats were divided into depression group and control group.Rats in depression group were exposed CUMS for 8 weeks consecutively.Weight,open-field test,sucrose preference,plasma concentration of noradrenaline and corticosterone were assayed before and after model establishment to evaluate the depression.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were measured by RT-qPCR and Western blotting,respectively. Results In CUMS induced depression group,weight,sucrose consumption,vertical score and horizontal score were decreased while levels of corticosterone and norepinephrine were increased compared with control group.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were reduced compared with control group. Conclusion The mRNA and protein expression of liver Oatps in rats were regulated down by CUMS induced depression.

LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
Citation: LIU Cuicui, XIAO Zhijun, LU Saihua, XU Feng. Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 121-125. doi: 10.3969/j.issn.1006-0111.2019.02.005
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