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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(1): 42-45,54

DONG Zhimin, XU Yueming, WEN Jun, JIANG Yiping, QIN Luping, XIN Hailiang. Study on fingerprint of Hupulus lupulus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010
Citation: DONG Zhimin, XU Yueming, WEN Jun, JIANG Yiping, QIN Luping, XIN Hailiang. Study on fingerprint of Hupulus lupulus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010
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Study on fingerprint of Hupulus lupulus by HPLC

doi: 10.3969/j.issn.1006-0111.2019.01.010
  • 1.

    College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China

  • 2.

    School of Pharmacy, Naval Medical University, Shanghai 200433, China

  • 3.

    College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China;College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China

  • Received Date: 2018-05-21
  • Rev Recd Date: 2018-10-08
  • Objective To establish fingerprint of Hupulus lupulus by HPLC. Methods Diamonsil C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid, in gradient elution mode, detection wavelength was 358 nm, column pressure at 10.2MPa, column temperature was 30℃, injection volume of 10 μl and flow rate was 1.0 ml/min. Results A common mode of HPLC fingerprint of Hupulus lupulus was established. 19 common peaks were calibrated, xanthohumol, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone for identification. Among the 26 batches of Hupulus lupulus, 5 batches of herbs had similarities of less than or equal 0.9, which were all wild varieties. Conclusion The method was fast, effective, accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus, which provided reference for quality control of Hupulus lupulus.
  • [1] ZANOLI P, ZAVATTI M. Pharmacognostic and pharmacological profile of Humulus lupulus L.[J].J Ethnopharmacol,2008, 116(3):383-396.
    [2] 国家中医药管理局《中华本草》编委会. 中华本草[M]. 上海:上海科学技术出版社, 2005.
    [3] HEYERICK A, VERVARCKE S, DEPYPERE H, et al. A first prospective,randomized, double-blind, placebo-controlled study on the use of a standardized hop extract to alleviate menopausal discomforts[J]. Maturitas,2006, 54, (2):164-175.
    [4] MILLIGAN S R, KALITA J C, POCOCK V, et al. The endocrine activities of 8-prenylnaringenin and related hop (Humulus lupulus L.) flavonoids[J].J Clin Endocrinol Metab, 2000, 85(12):4912-4915.
    [5] GERHAUSER C, ALT A, HEISS E, et al. Cancer chemopreventive activity of Xanthohumol, a natural product derived from hop[J]. Mol Cancer Ther, 2002, 1(11):959-969.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Study on fingerprint of Hupulus lupulus by HPLC

doi: 10.3969/j.issn.1006-0111.2019.01.010
  • 1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China
  • 2. School of Pharmacy, Naval Medical University, Shanghai 200433, China
  • 3. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China;College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China
  • Received Date: 2018-05-21
  • Rev Recd Date: 2018-10-08

Abstract: Objective To establish fingerprint of Hupulus lupulus by HPLC. Methods Diamonsil C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid, in gradient elution mode, detection wavelength was 358 nm, column pressure at 10.2MPa, column temperature was 30℃, injection volume of 10 μl and flow rate was 1.0 ml/min. Results A common mode of HPLC fingerprint of Hupulus lupulus was established. 19 common peaks were calibrated, xanthohumol, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone for identification. Among the 26 batches of Hupulus lupulus, 5 batches of herbs had similarities of less than or equal 0.9, which were all wild varieties. Conclusion The method was fast, effective, accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus, which provided reference for quality control of Hupulus lupulus.

DONG Zhimin, XU Yueming, WEN Jun, JIANG Yiping, QIN Luping, XIN Hailiang. Study on fingerprint of Hupulus lupulus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010
Citation: DONG Zhimin, XU Yueming, WEN Jun, JIANG Yiping, QIN Luping, XIN Hailiang. Study on fingerprint of Hupulus lupulus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010
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