Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

CHEN Hao; LI Xinfang; SUN Miao; ZHANG Gang; YANG Feng

doi:10.3969/j.issn.1006-0111.2019.01.009

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Objective To establish a method for concentration determination of garlic saponins PIEB in the rat plasma and study the pharmacokinetics of PIEB. Methods The separation was performed on a reverse phase Athena C₁₈-WP (50 mm×2.1 mm, 3 μm), with the mobile phase consisted of elution of acetonitrile-water (contain 0.05% formic acid) at a flow rate of 0.3 ml/min.After intravenous and gavage administration, the blood samples of rats were collected in different time, and measured by LC-MS/MS.The pharmacokinetics parameters were calculated by Inna Phase Kinetica2000^TM pharmacokinetics software. Results There was a good linearity of the calibration curves over the concentration range of 10-2 430 ng/ml (r=0.999 1), and the investigation of methodology all accorded with the demands.After intravenous administration of 50 mg/kg PIEB in rats, the maximum concentration of PIEB was achieved immediately.The t_1/2 of PIEB was about 322 minutes and the MRT was 270 minutes.After oral administration of low, medium and high concentrations of garlic saponin, the AUC and c_max increased with the increasing of dosage, which were consistent with the features of linear pharmacokinetics. Conclusion The established method was simple, accurate and sensitive, which could be suitable for the contents determination of garlic saponins PIEB in the rat plasma.

Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

1. Department of Pharmacy, Hainan Branch of PLA General Hospital, Sanya 572013, China

2. School of Pharmacy, Naval Medical University, Shanghai 200433, China

3. College of High Altitude Military Medicine, Army Medical University, Chongqing 400038, China

Received Date: 2018-10-06

Rev Recd Date: 2018-11-12

Key words:

Abstract: Objective To establish a method for concentration determination of garlic saponins PIEB in the rat plasma and study the pharmacokinetics of PIEB. Methods The separation was performed on a reverse phase Athena C₁₈-WP (50 mm×2.1 mm, 3 μm), with the mobile phase consisted of elution of acetonitrile-water (contain 0.05% formic acid) at a flow rate of 0.3 ml/min.After intravenous and gavage administration, the blood samples of rats were collected in different time, and measured by LC-MS/MS.The pharmacokinetics parameters were calculated by Inna Phase Kinetica2000^TM pharmacokinetics software. Results There was a good linearity of the calibration curves over the concentration range of 10-2 430 ng/ml (r=0.999 1), and the investigation of methodology all accorded with the demands.After intravenous administration of 50 mg/kg PIEB in rats, the maximum concentration of PIEB was achieved immediately.The t_1/2 of PIEB was about 322 minutes and the MRT was 270 minutes.After oral administration of low, medium and high concentrations of garlic saponin, the AUC and c_max increased with the increasing of dosage, which were consistent with the features of linear pharmacokinetics. Conclusion The established method was simple, accurate and sensitive, which could be suitable for the contents determination of garlic saponins PIEB in the rat plasma.

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Reference (10)

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Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

doi: 10.3969/j.issn.1006-0111.2019.01.009

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通讯作者: 陈斌, [email protected]

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