LU Mingxia, WANG Honggang, HU Renbiao. Effect of shikonin on expression of TGF-β1 in liver fibrosis induced by CCl4 in rats[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 453-456. doi: 10.3969/j.issn.1006-0111.2018.05.016
| Citation:
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LU Mingxia, WANG Honggang, HU Renbiao. Effect of shikonin on expression of TGF-β1 in liver fibrosis induced by CCl4 in rats[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(5): 453-456. doi: 10.3969/j.issn.1006-0111.2018.05.016
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Effect of shikonin on expression of TGF-β1 in liver fibrosis induced by CCl4 in rats
- 1.
JinHua People's Hospital, Jinhua 321000, China
- 2.
NingDe People's Hospital, Ningde 352000, China
- Received Date: 2018-03-15
- Rev Recd Date:
2018-05-10
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Abstract
Objective To observe the influence of shikonin on pathological changes of liver tissue, expression of TGF-β1 in liver fibrosis in rats and to explore the possible mechanism of shikonin on rats' liver fibrosis. Methods 40 SD rats were randomly divided into four groups:normal group,model group,positive controlled group and shikonin group. Except normal group, the liver fibrosis models were introduced by subcutaneously injecting CCl4. Meanwhile, The normal group and the model group were intragastrically given the distilled water. The positive controlled group was intragastrically given Compound Biejiaruangan Troche solution. The treatments lasted for ten weeks. After treatments, pathological changes were observed. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST)of serum were determined by automatic biochemical analyzer. The serum contents of laminin, procollagen type Ⅲ were assayed by ELISA. Transforming growth factor beta 1 (TGF-β1) index level was determined by immunohistochemistry. Results Compared with the normal group, liver fibrosis was detected in the model group. The serum levels of ALT,AST and the serum contents of laminin and procollagen type Ⅲ in the model group were significantly increased (P<0.05). Compared with the model group, the degree of liver fibrosis was less severe in shikonin group. The serum levels of ALT, AST, laminin, procollagen type Ⅲ and the expression of TGF-β1 in liver tissue were all decreased(P<0.05)in shikonin group. Compared with the positive controlled group, the expression of TGF-β1 in liver tissue was reduced in the shikonin group(P<0.05). Conclusion In SD rats, shikonin could inhibit the rats liver fibrosis induced with CCl4 by accommodating TGF-β1 expression in liver tissue.
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