ZHOU Qian, LIU Kui, MA Jing, TAN Guangguo. Protective effect of Qifu decoction on adriamycin-induced cardiac injury based on GC-MS serum metabolomics[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 313-317. doi: 10.3969/j.issn.1006-0111.2018.04.006
| Citation:
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ZHOU Qian, LIU Kui, MA Jing, TAN Guangguo. Protective effect of Qifu decoction on adriamycin-induced cardiac injury based on GC-MS serum metabolomics[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 313-317. doi: 10.3969/j.issn.1006-0111.2018.04.006
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Protective effect of Qifu decoction on adriamycin-induced cardiac injury based on GC-MS serum metabolomics
- 1.
Department of Traditional Chinese Medicine, Xijing Hospital, Air Force Medical University, Xi'an 710032, China
- 2.
Cadets Brigade, Basic Medicine College, Air Force Medical University, Xi'an 710032, China
- 3.
Department of Pharmaceutical Analysis, School of Pharmacy, Air Force Medical University, Xi'an 710032, China
- Received Date: 2018-05-12
- Rev Recd Date:
2018-05-28
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Abstract
Objective To evaluate the protective effect of Qifu decoction (QFD) on adriamycin-induced cardiac injury based on GC-MS serum metabolomics and explore its mechanism. Methods Twenty-four rats were randomly divided into three groups:control group, Adriamycin-induced model group and QFD-treated group. The QFD-treated group received QFD for 4 weeks. After 4 weeks, the serum samples of each rat were collected and analyzed by GC-MS. Combination GC-MS with multivariate and univariate statistical analysis was applied to identify potential biomarkers related with adriamycin-induced cardiac injury and QFD-reversed biomarkers. Metabolic pathway analysis was employed to identify QFD-targeted metabolic pathways. Results 17 metabolites were identified as potential biomarkers related with adriamycin-induced cardiac injury based on GC-MS serum metabolomics analysis and 10 metabolites were significantly reversed by QFD. QFD-targeted metabolic pathways were identified by metabolic pathway analysis with MetaboAnalyst, which were citrate cycle, glyoxylate and dicarboxylate metabolism and arachidonic acid metabolism. Conclusion QFD administration provided protective effects on adriamycin-induced cardiac injury through partially regulating the perturbed citrate cycle, glyoxylate and dicarboxylate metabolism and arachidonic acid metabolism.
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