Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia

MA Huiping; ZHANG Jun; JIA Zhengping; MENG Panpan; JING Linlin; WANG Rong

doi:10.3969/j.issn.1006-0111.2018.03.014

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To investigate anti-hypoxia activity and protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia. Methods The anti-hypoxic activity of Lishukang capsule was evaluated with normobaric hypoxia test and acute hypobaric hypoxia test in mice. In addition, rats were exposed to large hypobaric hypoxia chamber stimulating 8 000 m altitude. The pathological changes of rat brain tissue before and after hypoxia were observed. The oxidative stress indicators and metabolism parameters in brain were measured. Results The low, medium and high Lishukang doses can effectively prolong the survival time of mice (P<0.01) in the dose dependent manner. The medium and high Lishukang doses were significantly better than those of Rhodiola rosea capsules (P<0.05 or P<0.01). The low, medium, high Lishukang dose groups reduced the mortality of acute hypobaric hypoxia mice (P<0.01) with dose dependent effects. The mice mortality in medium and high dose groups was lower than that of Rhodiola rosea group (P<0.01). Compared with normal control group, the hypoxic model rats exhibited pathological injury in the brain tissue after exposure to hypobaric hypoxia stimulating 8 000 m altitude. The contents of MDA, H₂O₂, NO, LD and LDH activity increased significantly (P<0.05 or P<0.01), while the activities of SOD, CAT, GPX were significantly decreased (P<0.05 or P<0.01). After pretreatment with Lishukang capsule, the pathological damage of rat brain tissue was alleviated and the content of MDA, NO in the brain tissue was reduced (P<0.05 or P<0.01). The levels of H₂O₂, LD content and LDH activity in medium and high dose groups were significantly decreased (P<0.05 or P<0.01). The activities of SOD, CAT and GPX in high dose group were significantly increased (P<0.05). Conclusion Lishukang capsule has good anti-hypoxia activity. It provides protective effect for the injuries induced by hypobaric hypoxia in rats. The mechanism may related to the improvement of antioxidant capability, reduction of free radical damage and amelioration of energy metabolism.

Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia

Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China

Received Date: 2017-09-14

Rev Recd Date: 2018-01-24

Key words:

Abstract: Objective To investigate anti-hypoxia activity and protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia. Methods The anti-hypoxic activity of Lishukang capsule was evaluated with normobaric hypoxia test and acute hypobaric hypoxia test in mice. In addition, rats were exposed to large hypobaric hypoxia chamber stimulating 8 000 m altitude. The pathological changes of rat brain tissue before and after hypoxia were observed. The oxidative stress indicators and metabolism parameters in brain were measured. Results The low, medium and high Lishukang doses can effectively prolong the survival time of mice (P<0.01) in the dose dependent manner. The medium and high Lishukang doses were significantly better than those of Rhodiola rosea capsules (P<0.05 or P<0.01). The low, medium, high Lishukang dose groups reduced the mortality of acute hypobaric hypoxia mice (P<0.01) with dose dependent effects. The mice mortality in medium and high dose groups was lower than that of Rhodiola rosea group (P<0.01). Compared with normal control group, the hypoxic model rats exhibited pathological injury in the brain tissue after exposure to hypobaric hypoxia stimulating 8 000 m altitude. The contents of MDA, H₂O₂, NO, LD and LDH activity increased significantly (P<0.05 or P<0.01), while the activities of SOD, CAT, GPX were significantly decreased (P<0.05 or P<0.01). After pretreatment with Lishukang capsule, the pathological damage of rat brain tissue was alleviated and the content of MDA, NO in the brain tissue was reduced (P<0.05 or P<0.01). The levels of H₂O₂, LD content and LDH activity in medium and high dose groups were significantly decreased (P<0.05 or P<0.01). The activities of SOD, CAT and GPX in high dose group were significantly increased (P<0.05). Conclusion Lishukang capsule has good anti-hypoxia activity. It provides protective effect for the injuries induced by hypobaric hypoxia in rats. The mechanism may related to the improvement of antioxidant capability, reduction of free radical damage and amelioration of energy metabolism.

HTML

Reference (9)

[1]	郭晓敏,程捷恺,张明,等. UHPLC/MSn质谱树技术与Q/TOF联用在利舒康胶囊成分鉴定中的应用[J]. 中草药,2017,48(10):1944-1956.
[2]	Ma HP,Fan PC,Jing LL,et al. Anti-hypoxic activity at simulated high altitude was isolated in petroleum ether extract of Saussurea involucrata[J]. J. Ethnopharmacol, 2011, 137(3):1510-1515.
[3]	Maiti P, Singh SB, Sharma AK, et al. Hypobaric hypoxia induces oxidative stress in rat brain[J]. Neurochem Int,2006,49(8):709-716.
[4]	景临林,马慧萍,樊鹏程,等.氮氧自由基对高原缺氧小鼠脑组织中缺氧和凋亡蛋白的影响[J].中国医院药学杂志,2015,35(22):1986-1988.
[5]	Maity C, Lahiri P, Adak A, et al. Hypobaric-hypoxia induces alteration in microbes and microbes-associated enzyme profile in rat colonic samples[J]. Biomed Environ Sci,2013,26(10):869-873.
[6]	马慧萍,焦阳,高荣敏,等.大苞雪莲乙醇提取物对模拟高原缺氧小鼠生化指标的影响[J].中药材,2014,37(1):103-106.
[7]	Qian J, Jiang F,Wang B, et al. Ophiopogonin D prevents H₂O₂-induced injury in primary human umbilical vein endothelial cells[J]. J Ethnopharmacol, 2010, 128(2):438-445.
[8]	Shen ZB,Yin YQ,Tang CP, et al. Pharmacodynamic screening and simulation study of anti-hypoxia active fraction of Xiangdan injection[J]. J Ethnopharmacol, 2010,127(1):103-107.
[9]	Bailey DM,Bärtsch P, Knauth M, et al. Emerging concepts in acute mountain sickness and high-altitude cerebral edema:from the molecular to the morphological[J]. Cell Mol Life Sci, 2009, 66(22):3583-3594.

Message Board

Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia

doi: 10.3969/j.issn.1006-0111.2018.03.014

Abstract

References

Proportional views

通讯作者: 陈斌, [email protected]

Article Metrics

Related

Proportional views