Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

HUANG Jin; LIU Jingxue; ZENG Ying; YUAN Fang

doi:10.3969/j.issn.1006-0111.2018.02.007

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Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions. Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA. Cell proliferations were detected by CCK-8 experiment. RT-PCR test was used to detect the expression of oncogenes Akt2 and K-ras. Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pancreatic cancer cells. EGFR protein was increased by the interference of TRPV1 expression. The proliferation rate of pancreatic cancer cells was decreased and Akt2, K-ras were significantly inhibited by TRPV1 over-expressing. Conclusion The expression of TRPV1 in pancreatic cancer cells regulated the EGFR protein content. The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.

Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

1. Department Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201200, China;Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 200437, China

2. Department Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201200, China

3. Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 200437, China

Received Date: 2017-12-01

Rev Recd Date: 2018-01-04

Key words:

transient receptor potential vanilloid 1 /
EGFR /
pancreatic cancer cells /
cell proliferation

Abstract: Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions. Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA. Cell proliferations were detected by CCK-8 experiment. RT-PCR test was used to detect the expression of oncogenes Akt2 and K-ras. Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pancreatic cancer cells. EGFR protein was increased by the interference of TRPV1 expression. The proliferation rate of pancreatic cancer cells was decreased and Akt2, K-ras were significantly inhibited by TRPV1 over-expressing. Conclusion The expression of TRPV1 in pancreatic cancer cells regulated the EGFR protein content. The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.

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Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

doi: 10.3969/j.issn.1006-0111.2018.02.007

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通讯作者: 陈斌, [email protected]

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