Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO

WANG Jie; HUANG Yan; ZHANG Chuan; MA Xingyuan; CHANG Wenjun; LU Yiming

doi:10.3969/j.issn.1006-0111.2017.06.008

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Objective To investigate the effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO. Methods The human colorectal cancer cell line RKO of logarithmic growth phase was cultured 48 h with 5-FU. The expression of SHP2 was observed by immunofluorescence and Western blotting; siSHP2 which specifically inhibits the expression of SHP2 was transfected into RKO cells and cultured 48 h with 5-FU. The cell absorbance (A) values were measured with CCK8 and apoptosis was determined by flow cytometry. The sensitivity of RKO to 5-FU and the effect of 5-FU on apoptosis of RKO cells were observed. Results The expression of nuclear protein of SHP2 was improved remarkably after colorectal cancer cell RKO was cultured 48 h with 5-FU. The sensitivity of RKO cells to 5-FU and the cell apoptosis rate induced by 5-FU were decreased after siSHP2 transfection. Conclusion 5-FU exerts anti-cancer activity possibly due to its promoting the apoptosis of RKO cells by influencing the expression of SHP2.

Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO

1. Department of Biochemistry Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. College of Biological Engineering, East China University of Science and Technology, Shanghai 200237, China

3. Department of Environmental Hygiene, School of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China

Received Date: 2017-06-19

Rev Recd Date: 2017-07-10

Key words:

Abstract: Objective To investigate the effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO. Methods The human colorectal cancer cell line RKO of logarithmic growth phase was cultured 48 h with 5-FU. The expression of SHP2 was observed by immunofluorescence and Western blotting; siSHP2 which specifically inhibits the expression of SHP2 was transfected into RKO cells and cultured 48 h with 5-FU. The cell absorbance (A) values were measured with CCK8 and apoptosis was determined by flow cytometry. The sensitivity of RKO to 5-FU and the effect of 5-FU on apoptosis of RKO cells were observed. Results The expression of nuclear protein of SHP2 was improved remarkably after colorectal cancer cell RKO was cultured 48 h with 5-FU. The sensitivity of RKO cells to 5-FU and the cell apoptosis rate induced by 5-FU were decreased after siSHP2 transfection. Conclusion 5-FU exerts anti-cancer activity possibly due to its promoting the apoptosis of RKO cells by influencing the expression of SHP2.

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Reference (19)

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Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO

doi: 10.3969/j.issn.1006-0111.2017.06.008

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