Determination of pueratin in Jiangzhi Hugan capsule by HPLC

ZHENG Wei; NI Dongjie; WEI Qing; XIANG Hui; HU Lanlan; SUN Liang

doi:10.3969/j.issn.1006-0111.2017.03.016

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Objective To develop a HPLC method for determination of pueratin. Methods The separation was carried out on a Waters Symmetry C₁₈ column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 1% formic acid(11:89), the detection wavelength was set at 250 nm, the flow rate was 1.0 ml/min, the column temperature was 30 ℃ and the injection volume was 10 μl. Results The linearity was obtained over 2~40 μg/ml (r=0.999 8) for pueratin. The RSD of precision were less than 2%. The average recovery was between 98% and 103%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Jiangzhi Hugan capsule.

Determination of pueratin in Jiangzhi Hugan capsule by HPLC

No. 98 Hospital of PLA, Huzhou 313000, China

Received Date: 2015-03-13

Rev Recd Date: 2015-07-12

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Abstract: Objective To develop a HPLC method for determination of pueratin. Methods The separation was carried out on a Waters Symmetry C₁₈ column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 1% formic acid(11:89), the detection wavelength was set at 250 nm, the flow rate was 1.0 ml/min, the column temperature was 30 ℃ and the injection volume was 10 μl. Results The linearity was obtained over 2~40 μg/ml (r=0.999 8) for pueratin. The RSD of precision were less than 2%. The average recovery was between 98% and 103%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Jiangzhi Hugan capsule.

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Determination of pueratin in Jiangzhi Hugan capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2017.03.016

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通讯作者: 陈斌, [email protected]

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