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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(3): 224-228

ZHANG Jinyu, WANG Lihong, QIN Yulin, ZHANG Lulu, JIANG Yuanying, CAO Yongbing. Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 224-228. doi: 10.3969/j.issn.1006-0111.2017.03.008
Citation: ZHANG Jinyu, WANG Lihong, QIN Yulin, ZHANG Lulu, JIANG Yuanying, CAO Yongbing. Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 224-228. doi: 10.3969/j.issn.1006-0111.2017.03.008
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Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans

doi: 10.3969/j.issn.1006-0111.2017.03.008
  • 1.

    Research and Develop Center of New Drug, School of Pharmacy; Second Military Medical University, Shanghai 200433, China

  • 2.

    Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China

  • Received Date: 2017-02-28
  • Rev Recd Date: 2017-05-03
  • Objective To detect the transcription factors of copper ion (Cu2+) metabolism and oxidative stress by Candida albicans knocked down different transcription factors. Methods Spot assay, growth curve were used. Results The sensitivity to Cu2+ in Cup2Δ/Δ was increasing and the growth of Cup2Δ/Δ was inhibited in 5 mmol /L Cu2+ medium. The results showed that Cup2Δ/Δ also increased the sensitivity to H2O2, interestingly, Cu2+and H2O2 played a synergistic antifungal effect.The tolerance of Cup2Δ/Δ and SN250 to H2O2 induced oxidative stress was increased after BCS chelating Cu2+. In the fluconazole, miconazole and ketoconazole susceptibility experiments, Cup2Δ/Δ did not show susceptibility to azole drugs. Conclusion Knockout transcription factor Cup2, which could increase the sensitivity to Cu2+ and H2O2in Candida albicans. Transcription factor Cup2 might be involved in the regulation and control of Candida albicansmetabolism on Cu2+ and oxidative stress induced by H2O2, but not involved in the regulation and control of drug resistance to azole drugs.
  • [1] 杨雪梅,温洁新,李素华,等. 2010年我院124株白念珠菌药物敏感试验及耐药性分析[J]. 山西医药杂志,2012,41(5):445-446.
    [2] Kanoshiki RL, de Paula SB, Santos JP, et al. Effects of fluconazole treatment of mice infected with fluconazole-susceptible and -resistant Candida tropicalis on fungal cell surface hydrophobicity, adhesion and biofilm formation[J]. Indian J Med Microbiol,2015,33(Suppl):97-101.
    [3] 金仕强,杨 丁,左之才.硫酸铜联合聚维酮碘治疗育肥牛皮肤真菌感染一例[J]. 黑龙江畜牧兽医,2015,(11):130.
    [4] Dollwet, HHA., Sorenson, JRJ. Historic uses of copper compounds in medicine[J]. Trace Elem Med,1985,2 (2),80-87.
    [5] 木合塔尔. 应用煅硫酸铜治疗牛羊腐蹄病[J]. 中国兽医杂志,2002, (2):38
    [6] 张玉聚,宋凤仙. 高分子有机酸复合碱式硫酸铜的杀菌效果研究[J]. 华北农学报,1998,13(2):98-101.
    [7] Chudzik B, Tracz IB, Czernel G, et al. Amphotericin B-copper(Ⅱ) complex as a potential agent with higher antifungal activity against Candida albicans[J]. Eur J Pharm Sci,2013,49(5):850-857.
    [8] Goldstein S, Czapski G. Transition metalions and oxygen radicals[J]. Int Rev Exp Pathol,1990,31,133-164.
    [9] Kadiiska M B, Mason RP. In vivo copper-mediated free radical production: an ESR spin-trapping study[J]. Spectrochim Acta A Mol Biomol Spectrosc, 2002,58(6):1227-1239.
    [10] Flannagan RS, Cosío G, Grinstein S. Antimicrobial mechanisms of phagocytes and bacterial evasion strategies [J].Nat Rev Microbiol,2009,7(5):355-366.
    [11] Babu U, Failla ML. Respiratory burst and candidacidal activity of peritoneal macrophages are impaired in copper-deficient rats[J]. Nutr,1990,120 (12),1692-1699.
    [12] Buchman C, Skroch P, Welch J, et al. TheCup2 gene product, regulator of yeast metallothionein expression, is a copper-activated DNA-binding protein[J]. Mol Cell Biol,1989,9(9):4091-4095.
    [13] Hoffman AE, Miles L, Greenfield TJ, et al. Clinical isolates of Candida albicans, Candida tropicalis, and Candida krusei have different susceptibilities to Co(Ⅱ) and Cu(Ⅱ) complexes of 1,10-phenanthroline[J]. Bio Metals, 2015,28(2):415-423.
    [14] Jensen LT, Howard WR, Strain JJ, et al. Enhanced effectiveness of copper ion buffering by Cup 1 metallothionein compared with CRS5 metallothionein in Saccharomyces cerevisiae[J]. J Biol Chem,1996,271(31):18514-18519.
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Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans

doi: 10.3969/j.issn.1006-0111.2017.03.008
  • 1. Research and Develop Center of New Drug, School of Pharmacy; Second Military Medical University, Shanghai 200433, China
  • 2. Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China
  • Received Date: 2017-02-28
  • Rev Recd Date: 2017-05-03

Abstract: Objective To detect the transcription factors of copper ion (Cu2+) metabolism and oxidative stress by Candida albicans knocked down different transcription factors. Methods Spot assay, growth curve were used. Results The sensitivity to Cu2+ in Cup2Δ/Δ was increasing and the growth of Cup2Δ/Δ was inhibited in 5 mmol /L Cu2+ medium. The results showed that Cup2Δ/Δ also increased the sensitivity to H2O2, interestingly, Cu2+and H2O2 played a synergistic antifungal effect.The tolerance of Cup2Δ/Δ and SN250 to H2O2 induced oxidative stress was increased after BCS chelating Cu2+. In the fluconazole, miconazole and ketoconazole susceptibility experiments, Cup2Δ/Δ did not show susceptibility to azole drugs. Conclusion Knockout transcription factor Cup2, which could increase the sensitivity to Cu2+ and H2O2in Candida albicans. Transcription factor Cup2 might be involved in the regulation and control of Candida albicansmetabolism on Cu2+ and oxidative stress induced by H2O2, but not involved in the regulation and control of drug resistance to azole drugs.

ZHANG Jinyu, WANG Lihong, QIN Yulin, ZHANG Lulu, JIANG Yuanying, CAO Yongbing. Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 224-228. doi: 10.3969/j.issn.1006-0111.2017.03.008
Citation: ZHANG Jinyu, WANG Lihong, QIN Yulin, ZHANG Lulu, JIANG Yuanying, CAO Yongbing. Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(3): 224-228. doi: 10.3969/j.issn.1006-0111.2017.03.008
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