Determination of spinosin and total saponins in compound Zaoshen tablet by HPLC and UV

ZHENG Feng; QUE Xiao; SU Jing; DING Liyan; NIE Qu; QIU Mingfeng

doi:10.3969/j.issn.1006-0111.2017.02.009

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Objective To establish methods for the determination of spinosin and total saponins in compound Zaoshen tablet. Methods The separation of spinosin was performed on Agilent Zorbax SB C₁₈ column (250 mm×4.6 mm, 5 μm) at 30℃ with water and acetonitrile as mobile phase for gradient elution at the flow rate of 1.0 ml/min. The detection wavelength was 335 nm. Determination of total saponins was fulfilled by column chromatography followed by UV spectrometer with vanillin glacial acetic acid colorimetry. The detection wavelength was 560 nm. Results Linear range of spinosin was 20-100 μg/ml (r=0.999 0) and total saponins was 40-200 μg/ml (r=1.000 0). The average recoveries for accuracy tests were 99.55% and 99.85% respectively. Conclusion Those methods are accurate and reliable. They can be used for the quality control of compound Zaoshen tablet.

Determination of spinosin and total saponins in compound Zaoshen tablet by HPLC and UV

1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

2. Shanghai QiaoYuan Biological Pharmaceutical co., LTD., Shanghai 201203, China

3. School of Dai Medicine, West Yunnan University of Applied Sciences, Jinghong 600100, China

4. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;School of Dai Medicine, West Yunnan University of Applied Sciences, Jinghong 600100, China

Received Date: 2016-12-22

Rev Recd Date: 2017-02-28

Key words:

Abstract: Objective To establish methods for the determination of spinosin and total saponins in compound Zaoshen tablet. Methods The separation of spinosin was performed on Agilent Zorbax SB C₁₈ column (250 mm×4.6 mm, 5 μm) at 30℃ with water and acetonitrile as mobile phase for gradient elution at the flow rate of 1.0 ml/min. The detection wavelength was 335 nm. Determination of total saponins was fulfilled by column chromatography followed by UV spectrometer with vanillin glacial acetic acid colorimetry. The detection wavelength was 560 nm. Results Linear range of spinosin was 20-100 μg/ml (r=0.999 0) and total saponins was 40-200 μg/ml (r=1.000 0). The average recoveries for accuracy tests were 99.55% and 99.85% respectively. Conclusion Those methods are accurate and reliable. They can be used for the quality control of compound Zaoshen tablet.

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Determination of spinosin and total saponins in compound Zaoshen tablet by HPLC and UV

doi: 10.3969/j.issn.1006-0111.2017.02.009

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通讯作者: 陈斌, [email protected]

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