Determination of baicalin in Biyanling capsule by HPLC

GUO Liangjun; SUN Liang; ZHENG Wei; LU Ping; FU Mingqiang; GUO Jianbin

doi:10.3969/j.issn.1006-0111.2017.01.015

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Objective To develop a HPLC method for determination of baicalin. Methods The separation was carried out on a Waters XBridge C₁₈ column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 0.8% formic acid (25:75), the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the injection volume was 10 μl. Results The linearity was obtained over 1.25-40 μg/ml(r=0.999 9) for baicalin. The RSD of precision were less than 2%. The average recovery was between 95% and 100%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Biyanling capsule.

Determination of baicalin in Biyanling capsule by HPLC

No. 98 Hospital of PLA, Huzhou 313000, China

Received Date: 2015-03-13

Rev Recd Date: 2015-07-12

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Abstract: Objective To develop a HPLC method for determination of baicalin. Methods The separation was carried out on a Waters XBridge C₁₈ column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 0.8% formic acid (25:75), the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the injection volume was 10 μl. Results The linearity was obtained over 1.25-40 μg/ml(r=0.999 9) for baicalin. The RSD of precision were less than 2%. The average recovery was between 95% and 100%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Biyanling capsule.

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Reference (10)

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Determination of baicalin in Biyanling capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2017.01.015

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通讯作者: 陈斌, [email protected]

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