Study on quality standards of hospital preparation Ganmao granules

JIN Weihua; YU Botao; CHEN Hua; ZHANG Ming; FAN Kaihua; PU Zhiqiang; SONG Zonghui

doi:10.3969/j.issn.1006-0111.2016.06.013

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Objective To establish a quality standard for Ganmao granules of hospital preparations. Methods Radix Scutellariae, cortex phellodendri and radix bupleuri were identified by thin layer chromatography (TLC) qualitatively. High performance liquid chromatography (HPLC) was used for the content of baicalin. The determination was performed on Agilent HC-C₁₈ column (250 mm×4.6 mm,5 μm) at 30℃ with mobile phase composed of methanol -0.2% phosphoric acid (43:57) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm. Results In TLC chromatograms, the spectra of different test products had spots of the same color at corresponding sites, with no interference from negative control. A good linearity range of baicalin was 1.81~72.40 μg/ml (r=0.999 9). The average recovery rate was 98.55% (RSD=1.91%, n=9). Conclusion The quality standard was established and the method of identification has good reproducibility. The method of determination of baicalin content improved the controllability of formulated quality of Ganmao granules.

Study on quality standards of hospital preparation Ganmao granules

Department of Pharmacy, General Hospital of Chengdu Military Command, Chengdu 610083, China

Received Date: 2015-07-30

Rev Recd Date: 2016-04-28

Key words:

Ganmao granules /
baicalin /
TLC /
HPLC

Abstract: Objective To establish a quality standard for Ganmao granules of hospital preparations. Methods Radix Scutellariae, cortex phellodendri and radix bupleuri were identified by thin layer chromatography (TLC) qualitatively. High performance liquid chromatography (HPLC) was used for the content of baicalin. The determination was performed on Agilent HC-C₁₈ column (250 mm×4.6 mm,5 μm) at 30℃ with mobile phase composed of methanol -0.2% phosphoric acid (43:57) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm. Results In TLC chromatograms, the spectra of different test products had spots of the same color at corresponding sites, with no interference from negative control. A good linearity range of baicalin was 1.81~72.40 μg/ml (r=0.999 9). The average recovery rate was 98.55% (RSD=1.91%, n=9). Conclusion The quality standard was established and the method of identification has good reproducibility. The method of determination of baicalin content improved the controllability of formulated quality of Ganmao granules.

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Reference (9)

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Study on quality standards of hospital preparation Ganmao granules

doi: 10.3969/j.issn.1006-0111.2016.06.013

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