Effects of p-hydroxyacetophenone on bile secretion and blood lipid levels in rats

DENG Yifang; YU Pengxia; HUANG Xiaoling; HE Xingdong; YIN Ming; LIU Minyu

doi:10.3969/j.issn.1006-0111.2016.06.007

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Objective To study the effects and mechanism of p-hydroxyacetophenone (PHA) on bile secretion, cholesterol metabolism and blood lipids. Methods Cystic duct cannula was cannulated and bile were collected in SD rats.PHA was administrated in the high cholesterol rats. The activity of HMG-CoA reductase was determined by spectrophotometry in reaction system. Samples of liver were obtained in experiment hypercholesterolemia rats and Real-time PCR test was conducted for AQP8, CYP7A1, OATP and NTCP. Results Secretion of bile were increased, cholesterol in bile were reduced and secretion of total bile acid were increased in rats by PHA. The level of serum cholesterol in hyperglycemia rat model was reduced significantly and inhibitory ability had been limited in the activity of HMG-CoA reeducates by PHA, but the gene transcription including AQP8, CYP7A1, OATP, and NTCP were promoted, which are related to function of excretion of biliary acid and transformation of cholesterol. Conclusion These results suggest that PHA, as a lead compound, might be of significance for further development for a bile secretory and lipid lowering agent.

Effects of p-hydroxyacetophenone on bile secretion and blood lipid levels in rats

1. China State Institute of Pharmaceutical Industry, Shanghai 201203 China

2. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China

Received Date: 2015-12-28

Rev Recd Date: 2016-05-30

Key words:

Abstract: Objective To study the effects and mechanism of p-hydroxyacetophenone (PHA) on bile secretion, cholesterol metabolism and blood lipids. Methods Cystic duct cannula was cannulated and bile were collected in SD rats.PHA was administrated in the high cholesterol rats. The activity of HMG-CoA reductase was determined by spectrophotometry in reaction system. Samples of liver were obtained in experiment hypercholesterolemia rats and Real-time PCR test was conducted for AQP8, CYP7A1, OATP and NTCP. Results Secretion of bile were increased, cholesterol in bile were reduced and secretion of total bile acid were increased in rats by PHA. The level of serum cholesterol in hyperglycemia rat model was reduced significantly and inhibitory ability had been limited in the activity of HMG-CoA reeducates by PHA, but the gene transcription including AQP8, CYP7A1, OATP, and NTCP were promoted, which are related to function of excretion of biliary acid and transformation of cholesterol. Conclusion These results suggest that PHA, as a lead compound, might be of significance for further development for a bile secretory and lipid lowering agent.

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Effects of p-hydroxyacetophenone on bile secretion and blood lipid levels in rats

doi: 10.3969/j.issn.1006-0111.2016.06.007

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通讯作者: 陈斌, [email protected]

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