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Volume 34 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(4): 351-353

SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Citation: SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
shu

Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2016.04.017
  • 1.

    Food and Drug Inspection Center of Tangshan, Tangshan 063020, China

  • 2.

    The Children's Hospital of Hebei Province, Shijiazhuang 050030, China

  • Received Date: 2016-03-21
  • Rev Recd Date: 2016-05-26
  • Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peiminine) in Xiaoer Baotaikang granules. Methods The analysis was performed on a Thermo C18 column (250 mm×4.6 mm, 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine(65:35:0.03), and the flow rate was 1.0 ml/min; ELSD parameters:the temperature of drift tube was 85℃ and the carrier gas flow rate was 2.2 L/min. Results The linear ranges of peimine and peiminine were 0.586~5.860 μg (r=0.9996) and 0.564~5.640 μg (r=0.9999), respectively. The average recovery rate for peimine and peiminine was 99.44% (RSD was 0.7%) and 98.56% (RSD was 1.0%),respectively. Conclusion s The method is sample,accurate and reproducible, which could be used for the quality control of Xiaoer Baotaikang granules.
  • [1] 俞滢,朱亚尔. HPLC-MS测定癃闭康泰片中贝母素甲、贝母素乙的含量[J].中国药学杂志,2006,41(13):1026-1028.
    [2] 国家药典委员会. 中华人民共和国药典2015年版一部[S]. 北京:中国医药科技出版社,2015:540-541.
    [3] 范蕾,王伟影. 可同时测定蛇胆川贝液中贝母素甲与贝母素乙含量的方法[J]. 中国药业,2012,21(2):41-42.
    [4] 李慧婷,王冰,韩荣春. HPLC-ELSD同时测定平贝母中贝母素甲和贝母素乙含量[J].现代中药研究与实践,2010,24(4):58-60.
    [5] 马双成,张树潘,鲁静,等. 高效液相色谱-蒸发光散射检测法测定鹤蟾片中贝母素甲和贝母素乙的含量[J]. 药物分析杂志,2008,28(1):118-120.
    [6] 薛燕,顾好粮. HPLC-ELSD法测定浙贝母中主要生物碱的含量[J]. 药学学报,2005,40(6):550-552.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2016.04.017
  • 1. Food and Drug Inspection Center of Tangshan, Tangshan 063020, China
  • 2. The Children's Hospital of Hebei Province, Shijiazhuang 050030, China
  • Received Date: 2016-03-21
  • Rev Recd Date: 2016-05-26

Abstract: Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peiminine) in Xiaoer Baotaikang granules. Methods The analysis was performed on a Thermo C18 column (250 mm×4.6 mm, 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine(65:35:0.03), and the flow rate was 1.0 ml/min; ELSD parameters:the temperature of drift tube was 85℃ and the carrier gas flow rate was 2.2 L/min. Results The linear ranges of peimine and peiminine were 0.586~5.860 μg (r=0.9996) and 0.564~5.640 μg (r=0.9999), respectively. The average recovery rate for peimine and peiminine was 99.44% (RSD was 0.7%) and 98.56% (RSD was 1.0%),respectively. Conclusion s The method is sample,accurate and reproducible, which could be used for the quality control of Xiaoer Baotaikang granules.

SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Citation: SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
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