Comparision two kinds of content determination method of mannatide for injection

JIN Weihua; CHEN Hua; ZHANG Ming; PU Zhiqiang

doi:10.3969/j.issn.1006-0111.2016.01.017

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Objective To compare two methods in the determination of mannatide for injection. Methods The contents were determined by spectrophotometry and HPLC method. Results In the samples determined by Spectrophotometric Determination,soluble in phenol solution and sulfuric acid, was detected at wavelength 490 nm, D-mannose derivatives linear concentration range is 10.2-51 μg/ml (r=0.999 1), the average content was 92.14%, the average RSD value was 1.17%. In the HPLC determination, with color spectrum column: Gemini C₁₈ (250 mm×4.6 mm, 5 μm), mobile phase acetonitrile -0.02 mol/L ammonium acetate solution (20:80), flow rate:1 ml/min, the detection wavelength was at 250 nm, column temperature: 30 ℃, sample size: 10 μl detection. The average content ws 83.47%, the average RSD value was 0.65%, the content of two kinds of methods could effectively determine the D-mannose. Conclusion The average content for spectrophotometry was higher, but the phenol was used in detection of special odor, poisonous, corrosive, would cause a certain risk.HPLC was exclusive,chromatogram was more intuitive, on the operator harmfulness and less irritating. A new method for the determination of D-mannose was established at the same time.

Comparision two kinds of content determination method of mannatide for injection

Department of Pharmacy, Chengdu General Hospital of Chengdu Military Region, Chengdu 610083, China

Received Date: 2014-12-12

Rev Recd Date: 2015-04-14

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Abstract: Objective To compare two methods in the determination of mannatide for injection. Methods The contents were determined by spectrophotometry and HPLC method. Results In the samples determined by Spectrophotometric Determination,soluble in phenol solution and sulfuric acid, was detected at wavelength 490 nm, D-mannose derivatives linear concentration range is 10.2-51 μg/ml (r=0.999 1), the average content was 92.14%, the average RSD value was 1.17%. In the HPLC determination, with color spectrum column: Gemini C₁₈ (250 mm×4.6 mm, 5 μm), mobile phase acetonitrile -0.02 mol/L ammonium acetate solution (20:80), flow rate:1 ml/min, the detection wavelength was at 250 nm, column temperature: 30 ℃, sample size: 10 μl detection. The average content ws 83.47%, the average RSD value was 0.65%, the content of two kinds of methods could effectively determine the D-mannose. Conclusion The average content for spectrophotometry was higher, but the phenol was used in detection of special odor, poisonous, corrosive, would cause a certain risk.HPLC was exclusive,chromatogram was more intuitive, on the operator harmfulness and less irritating. A new method for the determination of D-mannose was established at the same time.

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Comparision two kinds of content determination method of mannatide for injection

doi: 10.3969/j.issn.1006-0111.2016.01.017

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通讯作者: 陈斌, [email protected]

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