HE Juan, ZHOU Chengjie. Study on pharmacokinetics and determination of actarit in human plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 41-43,75. doi: 10.3969/j.issn.1006-0111.2016.01.011
| Citation:
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HE Juan, ZHOU Chengjie. Study on pharmacokinetics and determination of actarit in human plasma by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 41-43,75. doi: 10.3969/j.issn.1006-0111.2016.01.011
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Study on pharmacokinetics and determination of actarit in human plasma by HPLC
- 1.
Department of Pharmacy, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
- 2.
Shanghai New Drug Research Center, Shanghai 201203, China
- Received Date: 2014-03-25
- Rev Recd Date:
2015-02-01
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Abstract
Objective To develop a simple and sensitive HPLC-UV method to determined actarit in plasma. Methods The chromatographic separation was carried out on a Dikma C18 column with a mobile phase composed of water containing 1.2% acetic acid-methanol (50:50, V/V) at a flow rate of 1.0 ml/min and the temperature of column was 30℃. UV wavelength mode was monitored at 245 nm. Results A linear response function was established for the range of concentrations 75-4 000 ng/ml for actarit. The inter-and intra-day precision value were less than 10% for actarit and the accuracy was more than 90%. The extraction recovery was more than 88%. Conclusion This simple, sensitive, and exclusive method could be used in routine clinical practice to monitor actarit concentrations in human plasma.
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