Effect of essential coptis decoction on rats with experimental ulcerative colitis

WU Tao; XIANG Fang; LIU Jing

doi:10.3969/j.issn.1006-0111.2016.01.008

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Objective To study the effect of coptis decoction on rats with ulcerative colitis. Methods Rat ulcerative colitis model was induced by 1,2-dimethylhydrazine(DMH)(40 mg/kg s.c.)+3% dextran sodium sulfate solution(DSS)(freely drinking). The drugs were given orally once a day for 7 day per week. After 5 weeks, the body weight, the number of colon ACFs and the number of colon cancer in rats, as well as the morphological changes of rat colon tissues were evaluated. Results Coptis decoction significantly improved the dyscrasia induced by DMH+DSS, the both of body weight and general condition were better than those of control group(P<0.05,P<0.01). Coptis decoction also significantly inhibited ACF in the rats treated by DMH+DSS for 10 weeks(P<0.01). The model group showed typical inflamed mucosa, a nearly complete subversion of the normal surface architecture. Compared with the model group, coptis decoction in each dosage group could improve rat atypical hyperplasia, mucosal hyperemia, edema and cells in the crowded. Transmission electron microscope showed that, in epithelial nuclei chromatin aggregation in the model group, the cell surface microvilli arranged disorder, fault phenomenon. Compared with model group, the coptis decoction had improved significantly. Conclusion Coptis decoction had significant therapeutic effect on rat ulcerative colitis.

Effect of essential coptis decoction on rats with experimental ulcerative colitis

1. Nanjing Pharmaceutical and Equipment Plant of Nanjing Military Region, Nanjing 210003, China

2. Department of Pharmacy, No.81 Hospital of PLA, Nanjing 210002, China

3. Department of Pharmacy, No. 454 Hospital of PLA, Nanjing 210002, China

Received Date: 2015-01-29

Rev Recd Date: 2015-07-25

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Abstract: Objective To study the effect of coptis decoction on rats with ulcerative colitis. Methods Rat ulcerative colitis model was induced by 1,2-dimethylhydrazine(DMH)(40 mg/kg s.c.)+3% dextran sodium sulfate solution(DSS)(freely drinking). The drugs were given orally once a day for 7 day per week. After 5 weeks, the body weight, the number of colon ACFs and the number of colon cancer in rats, as well as the morphological changes of rat colon tissues were evaluated. Results Coptis decoction significantly improved the dyscrasia induced by DMH+DSS, the both of body weight and general condition were better than those of control group(P<0.05,P<0.01). Coptis decoction also significantly inhibited ACF in the rats treated by DMH+DSS for 10 weeks(P<0.01). The model group showed typical inflamed mucosa, a nearly complete subversion of the normal surface architecture. Compared with the model group, coptis decoction in each dosage group could improve rat atypical hyperplasia, mucosal hyperemia, edema and cells in the crowded. Transmission electron microscope showed that, in epithelial nuclei chromatin aggregation in the model group, the cell surface microvilli arranged disorder, fault phenomenon. Compared with model group, the coptis decoction had improved significantly. Conclusion Coptis decoction had significant therapeutic effect on rat ulcerative colitis.

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Effect of essential coptis decoction on rats with experimental ulcerative colitis

doi: 10.3969/j.issn.1006-0111.2016.01.008

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通讯作者: 陈斌, [email protected]

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