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Volume 33 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(6): 518-521,575

WANG Keqi, Xu Weiheng, Ding Li, ZHANG Junping. The expression of protein kinase CK2α in rat hepatic fibrogenesis process[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 518-521,575. doi: 10.3969/j.issn.1006-0111.2015.06.010
Citation: WANG Keqi, Xu Weiheng, Ding Li, ZHANG Junping. The expression of protein kinase CK2α in rat hepatic fibrogenesis process[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 518-521,575. doi: 10.3969/j.issn.1006-0111.2015.06.010
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The expression of protein kinase CK2α in rat hepatic fibrogenesis process

doi: 10.3969/j.issn.1006-0111.2015.06.010

  • Department of Biochemic Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2015-06-10
  • Rev Recd Date: 2015-10-11
  • Objective To observe the dynamic characteristics of protein kinase, casein kinase II α (CK2α), expression during hepatic fibrogenesis in rats;and the effects of a matrine derivative, 13-methylamino-18-thione-matrine (MASM), on CK2α expression when it is used for anti-fibrotic treatment. Methods Hepatic fibrosis model was established in SD rats by dimethylnitrosamine (DMN) injection or by bile duct ligation (BDL). The established fibrotic rats were given 50 mg/kg MASM or saline as a control by gavage for three weeks. The level of hepatic fibrosis was evaluated by histopathology examination using hematoxylin-eosin staining, and using the sirius red and Masson's trichrome staining for collagen determination in fibrosis. The expressions of CK2α and α-smooth muscle actin (α-SMA) in hepatic tissues were detected by immunohistochemisry. Results CK2α is mainly expressed in the stellate cells of fibrotic livers induced by DMN or BDL comparing the control group. Along with the development of hepatic fibrosis as evidenced by α-SMA expression, increased CK2α-positive cells in liver were detected while injecting DMN in the rats for one to four weeks. MASM treatment significantly inhibited the hepatic fibrosis and suppressed the expression of CK2α comparing the model group. Conclusion The expression level of CK2α, and hepatic fibrosis formation are positively correlated. The matrine derivative, MASM, can significantly inhibit hepatic fibrosis and suppress the CK2α expression. These Results suggest CK2α may be a potential target for hepatic fibrosis therapy.
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    [2] Friedman SL. Mechanisms of hepatic fibrogenesis[J]. Gastroenterology, 2008, 134(6):1655-1669.
    [3] Rippe RA, Brenner DA. From quiescence to activation:gene regulation in hepatic stellate cells[J]. Gastroenterology, 2004, 127(4):1260-1262.
    [4] Cozza G, Bortolato A, Moro S. How druggable is protein kinase CK2 ?[J]. Med Res Rev, 2010, 30(3):419-62.
    [5] Wang D, Westerheide SD, Hanson JL, et al. Tumor necrosis factor alpha-induced phosphorylation of ReIA/p65 0n Ser529 is controlled by casein kinase Ⅱ[J]. J Biol Chem, 2000, 275(42):32592-32597.
    [6] Rodriguez FA. Contreras C, Bolanos-Garcia V, et al. Protein kinase CK2 as an ectokinase: the role of the regulatory CK2 beta subunit[J]. Proc Natl Acad Sci(USA), 2008, 105(15):5693-5698.
    [7] Prudent R, Cochet C. New protein kinase CK2 inhibitors: jumping out of the catalytic box[J]. Chem Biol, 2009, 16(2):112-120.
    [8] Shi D,Zhang J,Qiu L,et al.Matrine inhibits infiltration of the inflammatory gr1(hi) monocyte subset in injured mouse liver through inhibition of monocyte chemoattractant protein-1[J].Evid Based Compl Alternat Med,2013,2013:580673.
    [9] Wang W, You RL, Qin WJ, et al. Anti-tumor activities of active ingredients in compound Kushen injection[J]. Acta Pharmacol Sin, 2015, 36(6):676-679.
    [10] Yang Y, Xiu J, Zhang X, et al. Antiviral effect of matrine against human enterovirus 71[J]. Molecules, 2012, 17(9):10370-10376.
    [11] Zhang JP, Zhang M, Zhou JP, et al. Antifibrotic effects of matrine on in vitro and in vivo models of liver fibrosis in rats[J]. Acta Pharmacol Sin, 2001, 22(2):183-186.
    [12] Hu H,Wang S,Zhang C,et al.Synthesis and in vitro inhibitory activity of matrine derivatives towards pro-inflammatory cytokines[J].Bioorg Med Chem Lett,2010,20(24):7537-7539.
    [13] Xu WH, Hu HG, Tian Y, et al. Bioactive compound reveals a novel function for ribosomal protein S5 in hepatic stellate cell activation and hepatic fibrosis[J]. Hepatology, 2014, 60(2): 648-660.
    [14] Xu Y, Peng Z, Ji W, et al. A novel matrine derivative WM130 inhibits activation of hepatic stellate cells and attenuates dimethylnitrosamine-induced liver fibrosis in rats[J]. Biomed Res Int, 2015, 2015:203978.
    [15] Qian L, Liu Y, Xu Y, et al. Matrine derivative WM130 inhibits hepatocellular carcinoma by suppressing EGFR/ERK/MMP-2 and PTEN/AKT signaling pathways[J]. Cancer Lett, 2015, 368(1):126-134.
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The expression of protein kinase CK2α in rat hepatic fibrogenesis process

doi: 10.3969/j.issn.1006-0111.2015.06.010
  • Department of Biochemic Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2015-06-10
  • Rev Recd Date: 2015-10-11

Abstract: Objective To observe the dynamic characteristics of protein kinase, casein kinase II α (CK2α), expression during hepatic fibrogenesis in rats;and the effects of a matrine derivative, 13-methylamino-18-thione-matrine (MASM), on CK2α expression when it is used for anti-fibrotic treatment. Methods Hepatic fibrosis model was established in SD rats by dimethylnitrosamine (DMN) injection or by bile duct ligation (BDL). The established fibrotic rats were given 50 mg/kg MASM or saline as a control by gavage for three weeks. The level of hepatic fibrosis was evaluated by histopathology examination using hematoxylin-eosin staining, and using the sirius red and Masson's trichrome staining for collagen determination in fibrosis. The expressions of CK2α and α-smooth muscle actin (α-SMA) in hepatic tissues were detected by immunohistochemisry. Results CK2α is mainly expressed in the stellate cells of fibrotic livers induced by DMN or BDL comparing the control group. Along with the development of hepatic fibrosis as evidenced by α-SMA expression, increased CK2α-positive cells in liver were detected while injecting DMN in the rats for one to four weeks. MASM treatment significantly inhibited the hepatic fibrosis and suppressed the expression of CK2α comparing the model group. Conclusion The expression level of CK2α, and hepatic fibrosis formation are positively correlated. The matrine derivative, MASM, can significantly inhibit hepatic fibrosis and suppress the CK2α expression. These Results suggest CK2α may be a potential target for hepatic fibrosis therapy.

WANG Keqi, Xu Weiheng, Ding Li, ZHANG Junping. The expression of protein kinase CK2α in rat hepatic fibrogenesis process[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 518-521,575. doi: 10.3969/j.issn.1006-0111.2015.06.010
Citation: WANG Keqi, Xu Weiheng, Ding Li, ZHANG Junping. The expression of protein kinase CK2α in rat hepatic fibrogenesis process[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(6): 518-521,575. doi: 10.3969/j.issn.1006-0111.2015.06.010
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