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Volume 33 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(4): 356-358

LV Fang, ZHOU Jin, FEI Yang, CHEN Yu, ZHANG Guoqing, ZHAO Liang. Determination of asiatic acid in the root of Actinidia valvata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 356-358. doi: 10.3969/j.issn.1006-0111.2015.04.017
Citation: LV Fang, ZHOU Jin, FEI Yang, CHEN Yu, ZHANG Guoqing, ZHAO Liang. Determination of asiatic acid in the root of Actinidia valvata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 356-358. doi: 10.3969/j.issn.1006-0111.2015.04.017
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Determination of asiatic acid in the root of Actinidia valvata by HPLC

doi: 10.3969/j.issn.1006-0111.2015.04.017

  • Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China

  • Received Date: 2014-03-06
  • Rev Recd Date: 2014-09-09
  • Objective To establish a method for the determination of asiatic acid in the root of Actinidia valvata. Methods HPLC-VWD was used in the analysis. The column was Agilent HC-C18 (4.6 mm×250 mm,5μm);the mobile phase was acetonitrile: (30 mmol/L) acetic acid amine solution (35:65); the flow rate was 1.0 ml/min; the temperature of column was 25 ℃; the detection wavelength was set at 210 nm; the injection volume was 25 μl; the running time was 35 min. Results Asiatic acid was separated with interference in baseline. The linear range was 25.30-506.0 μg/ml with linear correlation of 0.999 6 for asiatic acid. The result of intra-day and inter-day precisions were both within 5% (n=3), and the average recovery was 99.4% with RSD 1.9% (n=6). Conclusion The method was simple, rapid, accurate and convenient for quality control of asiatic acid in the root of Actinidia valvata.
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    [3] 万旭英,张 晨,凌昌全,等. 猫人参注射液抗肝癌作用和对免疫功能的影响[J].浙江中医学院学报,2004,28 (4): 56-59.
    [4] 徐一新,项昭保,陈晓晶,等. 中药猫人参中的抗肿瘤活性成分[J].第二军医大学学报,2011,32(7): 749-753.
    [5] 吕 昉,赵 亮,郑 磊,等. HPLC-TOF/MS对中药猫人参化学成分的快速鉴别[J].中南药学,2014,(2): 165-168.
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    [8] 周 军,高 静,方春钱,等. 积雪草酸对肝癌细胞增殖作用的影响[J].江苏大学学报(医学版),2009,19(6): 475-479.
    [9] 汤丽霞,杨 光,谭家驹. 积雪草酸诱导大鼠肝星状细胞凋亡[J].中草药,2009,(S1): 230-232.
    [10] 夏彬彬,李伊莎,徐唯哲,等. 积雪草中积雪草酸的分离、纯化及其含量测定[J].首都医科大学学报,2011,32(04): 538-540.
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Determination of asiatic acid in the root of Actinidia valvata by HPLC

doi: 10.3969/j.issn.1006-0111.2015.04.017
  • Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China
  • Received Date: 2014-03-06
  • Rev Recd Date: 2014-09-09

Abstract: Objective To establish a method for the determination of asiatic acid in the root of Actinidia valvata. Methods HPLC-VWD was used in the analysis. The column was Agilent HC-C18 (4.6 mm×250 mm,5μm);the mobile phase was acetonitrile: (30 mmol/L) acetic acid amine solution (35:65); the flow rate was 1.0 ml/min; the temperature of column was 25 ℃; the detection wavelength was set at 210 nm; the injection volume was 25 μl; the running time was 35 min. Results Asiatic acid was separated with interference in baseline. The linear range was 25.30-506.0 μg/ml with linear correlation of 0.999 6 for asiatic acid. The result of intra-day and inter-day precisions were both within 5% (n=3), and the average recovery was 99.4% with RSD 1.9% (n=6). Conclusion The method was simple, rapid, accurate and convenient for quality control of asiatic acid in the root of Actinidia valvata.

LV Fang, ZHOU Jin, FEI Yang, CHEN Yu, ZHANG Guoqing, ZHAO Liang. Determination of asiatic acid in the root of Actinidia valvata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 356-358. doi: 10.3969/j.issn.1006-0111.2015.04.017
Citation: LV Fang, ZHOU Jin, FEI Yang, CHEN Yu, ZHANG Guoqing, ZHAO Liang. Determination of asiatic acid in the root of Actinidia valvata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 356-358. doi: 10.3969/j.issn.1006-0111.2015.04.017
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