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Volume 33 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(3): 235-237,241

YI Bo, ZHANG Min, LIN Hai, CHEN Tao, XU Wentong, FENG Shixiu. Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011
Citation: YI Bo, ZHANG Min, LIN Hai, CHEN Tao, XU Wentong, FENG Shixiu. Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011
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Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo

doi: 10.3969/j.issn.1006-0111.2015.03.011
  • 1.

    Department of Pharmacy, No. 187 Hospital of PLA, Haikou 571159, China

  • 2.

    Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China;College of Life Science, Northwest A&F University, Yangling 712100, China

  • 3.

    Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China

  • Received Date: 2013-10-27
  • Rev Recd Date: 2014-05-22
  • Objective To screen the hemostatic active extracts from Callicarpa nudiflora. Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H2O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H2O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days. Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H2O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.
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Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo

doi: 10.3969/j.issn.1006-0111.2015.03.011
  • 1. Department of Pharmacy, No. 187 Hospital of PLA, Haikou 571159, China
  • 2. Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China;College of Life Science, Northwest A&F University, Yangling 712100, China
  • 3. Key Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China
  • Received Date: 2013-10-27
  • Rev Recd Date: 2014-05-22

Abstract: Objective To screen the hemostatic active extracts from Callicarpa nudiflora. Methods The powdered Callicarpa nudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract. The EtOH-extract was further chromatographed over HP-20 macroporous resin column, eluting with aqua and 95% EtOH to get HP-H2O-elution and HP-EtOH-elution, respectively. The obtained three extracts of EtOH-extrract, HP-EtOH-elution and HP-H2O-elution were set as large, middle and small dosage groups for drug preparation, respectively. Yunnan Baiyao was used as a positive control group.The weight increment, bleeding time and clotting time of fed mice were detected by cutting tail and grass slide Methods after intragastric administration for 7 days. Results As compared with blank model and positive control, each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time, of which the small dosage group and middle dosage group even exhibited better Results than the positive control group.Whereas the EtOH-extract, HP-EtOH-elution and HP-H2O-elution didn't demonstrated significant effect on clotting time as well as the weight increment. Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages.The hemostatic mechanism may through intrinsic coagulation pathway.This study would be helpful for further phytochemical investigation of C.nudiflora.

YI Bo, ZHANG Min, LIN Hai, CHEN Tao, XU Wentong, FENG Shixiu. Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011
Citation: YI Bo, ZHANG Min, LIN Hai, CHEN Tao, XU Wentong, FENG Shixiu. Screening for the hemostatic active extracts of Callicarpa nudiflora, in mice in vivo[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 235-237,241. doi: 10.3969/j.issn.1006-0111.2015.03.011
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