WANG Yan, YU Zhongwang, CHEN Si, LI Ling, ZHU Zhenyu. Comparative analysis of different states of polarized BV2 cells by GC-MS combined with metabonomic technology[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 226-230. doi: 10.3969/j.issn.1006-0111.2015.03.009
| Citation:
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WANG Yan, YU Zhongwang, CHEN Si, LI Ling, ZHU Zhenyu. Comparative analysis of different states of polarized BV2 cells by GC-MS combined with metabonomic technology[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 226-230. doi: 10.3969/j.issn.1006-0111.2015.03.009
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Comparative analysis of different states of polarized BV2 cells by GC-MS combined with metabonomic technology
- 1.
Second Military Medical University, Department of Pharmaceutical Analysis, School of Pharmacy, Shanghai 200433, China
- 2.
Second Military Medical University Institute of Neuroscience and Key Laboratory of Molecular Neurobiology of the Ministry of Education, College of Basic Medical Science, Shanghai 200433, China
- 3.
Second Military Medical University Pharmaceutical Analysis Center, School of Pharmacy, Shanghai 200433, China
- Received Date: 2014-12-02
- Rev Recd Date:
2015-03-17
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Abstract
Objective To analyze the different metabolites of the classical activated(M1), alternatively activated(M2) and resting BV2 cells by metabolomics method. Methods The mRNAs of several potential biomarkers were determined by real-time PCR analyses to confirm the state of BV2 cells. Static GC-MS combined with metabolomics technology was used to analyze the metabolic changes. Results There were 15 biomarkers identified between the M1 group and the resting group, and 15 biomarkers were found in the M2 group and the resting group. Conclusion The present study provides an effective way to reveal the mechanism of the polarization of BV2 cell, and it might provide a theoretical basis to prevent or treat the neurodegenerative diseases.
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