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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(2): 159-162,192

WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
Citation: WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
shu

Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma

doi: 10.3969/j.issn.1006-0111.2015.02.018
  • 1.

    Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

  • 2.

    Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

  • 3.

    College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

  • 4.

    Department of Integration of Traditional Chinese and Western medicine, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

  • 5.

    Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

  • Received Date: 2014-11-01
  • Rev Recd Date: 2015-01-29
  • Objective To study the relationship between circulating tumor cells(CTCs) in peripheral blood of the patients with hepatocellular carcinoma (HCC) and its metastasis and prognosis. Methods 35 patients with hepatocellular carcinoma were collected as the research objects.CTCs density were enriched through gradient centrifugation and negative-immunomagnetic methods, then the cells were detected by chromosome fluorescence in situ hybridization combined with immunofluorescence tests to identify CTCs. The clinical characters were recorded and the data were statistically analyzed. Results All the patients were detected CTC positive. The number of CTC was (4.1±2.5).The patients were divided into 2 groups.Group Ⅰ included patients whose CTCs were <5,and others were included in group Ⅱ.The difference between number of group I and group Ⅱ had significant significance(P=0.001); Metastasis had nothing to do with patients' sex and age(P=0.581,0.531); The number of CTCs was related to metastasis and prognosis(P=0.024,0.01),and there was significant statistic significance between group I and group Ⅱ. Conclusion The number of CTCs was related to tumor metastasis.The tumor may be more prone to occur metastasis and may had worse prognosis,and the patients may had shorter life time when the number of CTCs was≥5.
  • [1] Cha C, Fong Y, Jarnagin WR, et al. Predictors and patterns of recurrence after resection of hepatocellular carcinoma[J]. J Am Coll Surg, 2003, 197(5): 753-758.
    [2] Pantel K, Brakenhoff RH. Dissecting the metastatic cascade[J]. Nat Rev Cancer, 2004, 4(6): 448-456.
    [3] Shao C, Liao CP, Hu P, et al. Detection of live circulating tumor cells by a class of near-infrared heptamethine carbocyanine dyes in patients with localized and metastatic prostate cancer[J]. PLoS One, 2014, 9(2): e88967.
    [4] Seeberg LT, Waage A, Brunborg C, et al. Circulating tumor cells in patients with colorectal liver metastasis predict impaired survival[J]. Ann Surg, 2015, 261(1): 164-171.
    [5] Mikulova V, Cabinakova M, Janatkova I, et al. Detection of circulating tumor cells during follow-up of patients with early breast cancer: clinical utility for monitoring of therapy efficacy[J]. Scand J Clin Lab Invest, 2014, 74(2): 132-142.
    [6] Muinelo-Romay L, Vieito M, Abalo A, et al. Evaluation of circulating tumor cells and related events as prognostic factors and surrogate biomarkers in advanced NSCLC patients receiving first-line systemic treatment[J]. Cancers (Basel), 2014, 6(1): 153-165.
    [7] Poveda A, Kaye SB, McCormack R, et al. Circulating tumor cells predict progression free survival and overall survival in patients with relapsed/recurrent advanced ovarian cancer[J]. Gynecol Oncol, 2011, 122(3): 567-572.
    [8] Zigeuner RE, Riesenberg R, Pohla H, et al. Isolation of circulating cancer cells from whole blood by immunomagnetic cell enrichment and unenriched immunocytochemistry in vitro[J]. J Urol, 2003, 169(2): 701-705.
    [9] Wu C, Hao H, Li L, et al. Preliminary investigation of the clinical significance of detecting circulating tumor cells enriched from lung cancer patients[J]. J Thorac Oncol, 2009, 4(1): 30-36.
    [10] Gisselsson D.Refined characterisation of chromosome aberrations in tumours by multicolour banding and electronic mapping resources[J].Methods Cell Sci,2001,23(1-3):23-28.
    [11] Ashworth TR. A case of cancer in which cells similar to those in the tumors were seen in the blood after death[J]. Aust Med J, 1869, 14(146-149.
    [12] Yu M, Bardia A, Wittner BS, et al. Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition[J]. Science, 2013, 339(6119): 580-584.
    [13] Labelle M, Begum S, Hynes RO. Direct signaling between platelets and cancer cells induces an epithelial-mesenchymal-like transition and promotes metastasis[J]. Cancer Cell, 2011, 20(5): 576-590.
    [14] 曹宇勃,许崇安. 循环肿瘤细胞预测肝细胞癌患者临床预后价值的Meta分析[J]. 中国全科医学, 2014, 17(12): 1394-1399.
    [15] Sun YF, Xu Y, Yang XR, et al. Circulating stem cell-like epithelial cell adhesion molecule-positive tumor cells indicate poor prognosis of hepatocellular carcinoma after curative resection[J]. Hepatology, 2013, 57(4): 1458-1468.
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Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma

doi: 10.3969/j.issn.1006-0111.2015.02.018
  • 1. Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
  • 2. Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
  • 3. College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
  • 4. Department of Integration of Traditional Chinese and Western medicine, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
  • 5. Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
  • Received Date: 2014-11-01
  • Rev Recd Date: 2015-01-29

Abstract: Objective To study the relationship between circulating tumor cells(CTCs) in peripheral blood of the patients with hepatocellular carcinoma (HCC) and its metastasis and prognosis. Methods 35 patients with hepatocellular carcinoma were collected as the research objects.CTCs density were enriched through gradient centrifugation and negative-immunomagnetic methods, then the cells were detected by chromosome fluorescence in situ hybridization combined with immunofluorescence tests to identify CTCs. The clinical characters were recorded and the data were statistically analyzed. Results All the patients were detected CTC positive. The number of CTC was (4.1±2.5).The patients were divided into 2 groups.Group Ⅰ included patients whose CTCs were <5,and others were included in group Ⅱ.The difference between number of group I and group Ⅱ had significant significance(P=0.001); Metastasis had nothing to do with patients' sex and age(P=0.581,0.531); The number of CTCs was related to metastasis and prognosis(P=0.024,0.01),and there was significant statistic significance between group I and group Ⅱ. Conclusion The number of CTCs was related to tumor metastasis.The tumor may be more prone to occur metastasis and may had worse prognosis,and the patients may had shorter life time when the number of CTCs was≥5.

WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
Citation: WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
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