Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD

BI Xueyan; HU Pan; ZHANG Shengbo; ZHANG Qingbo

doi:10.3969/j.issn.1006-0111.2015.02.016

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Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its uniformity and stability. Methods UPLC-ELSD was applied to obtain a chromatogram with 11 well separated peaks, and the chromatographic condition was as follows: mobile phase was gradient elution by acetonitrile-water, column was ACQUITY UPLC BEH C₁₈ (2.1×100 mm, 1.7 μm) with the column temperature of 30 ℃ and the flow rate of 0.2 ml/min. Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. Similarities were all greater than 0.90, which could meet the requirement for chromatographic fingerprint of TCM. Conclusion The stability, repeatability and precision of the developed method were all validated, and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine.

Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD

1. Heilongjiang Institute for Food and Drug Control, Harbin 150001, China

2. Anshan Institute for Drug Control, Anshan 114000, China

3. Jining Institute for Drug Control, Jining 272025, China

Received Date: 2014-01-04

Rev Recd Date: 2014-07-18

Key words:

Platycodonis /
liquid extract /
UPLC /
ELSD /
fingerprint

Abstract: Objective To develop a stable method for fingerprint analysis of extractum platycodi liquidum to ensure its uniformity and stability. Methods UPLC-ELSD was applied to obtain a chromatogram with 11 well separated peaks, and the chromatographic condition was as follows: mobile phase was gradient elution by acetonitrile-water, column was ACQUITY UPLC BEH C₁₈ (2.1×100 mm, 1.7 μm) with the column temperature of 30 ℃ and the flow rate of 0.2 ml/min. Results The chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. Similarities were all greater than 0.90, which could meet the requirement for chromatographic fingerprint of TCM. Conclusion The stability, repeatability and precision of the developed method were all validated, and the chromatographic fingerprint analysis was a rapid and accurate method for the quality evaluation of traditional Chinese medicine.

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Reference (8)

[1]	中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准[S].第11册.1996:149.
[2]	许传莲,郑毅男,杨腊虎,等.HPLC法测定不同采收期及不同部位桔梗中桔梗皂苷D含量[J]. 吉林农业大学学报,2001,23(1):58-60,64.
[3]	朱丹妮,舒娈,郅慧,等.HPLC-ELSD法测定桔梗中桔梗皂苷D的含量[J]. 植物资源与环境学报,2001,10(4):11-13.
[4]	许传莲,杨腊虎,郑毅男,等.应用RP-HPLC法测定不同产地桔梗中桔梗皂苷D[J]. 吉林农业大学学报,1999,21(4):35-38.
[5]	黄樱,史春蕾,刘墨祥,等.HPLC-ELSD法测定桔梗饮片中8种桔梗皂苷的含量[J]. 扬州大学学报(自然科学版),2008,11(4):41-44.
[6]	石俊英,王颖,巩丽丽,等.HPLC法测定桔梗不同部位、不同产地药材中桔梗皂苷D含量[J]. 山东中医药大学学报,2007,31(6):501-503.
[7]	李喜凤,刘素梅,李振国.RP-HPLC法测定桔梗中桔梗皂苷D的含量[J]. 中华中医药学刊,2008,26(2):283-284.
[8]	李文庭,祝明,马临科,等. 桔梗的HPLC-ELSD指纹图谱研究[J]. 中国实验方剂学杂志,2011,17(22):50-53.

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Research on fingerprint analysis of platycodonis liquid extract by UPLC-ELSD

doi: 10.3969/j.issn.1006-0111.2015.02.016

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