HAN Lin, LV Chao, LI Min, HUANG Huimei, CHANG Wanlin, PENG Chengcheng, LIU Runhui. Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012
| Citation:
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HAN Lin, LV Chao, LI Min, HUANG Huimei, CHANG Wanlin, PENG Chengcheng, LIU Runhui. Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012
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Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill
- 1.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 2.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, China
- 3.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
- Received Date: 2014-01-15
- Rev Recd Date:
2014-04-02
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Abstract
Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill (SBP) absorbed in blood against hypoxia/reoxygenation injury. Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats(1~3 days old) and were used to build hypoxia/reoxygenation injury model. The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method. Results SBP showed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury atthe concentration of 50 μg/ml. Ginsenoside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury. Conclusion SBP have the protective activity against cardiomyocytes hypoxia/reoxygenation injury, and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP. This experiment offered basis for further pharmacodynamics and mechanism study of SBP.
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