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Volume 32 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2014  >  32(1): 56-58,67

YANG Wenhui, XIAO Qiusheng, JIAG Zongwen, ZHANG Su, SONG Haiyan, Wang Dianrong. In vitro release of thermosensitive slow-release rectal liquid suppository of insulin[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 56-58,67. doi: 10.3969/j.issn.1006-0111.2014.01.016
Citation: YANG Wenhui, XIAO Qiusheng, JIAG Zongwen, ZHANG Su, SONG Haiyan, Wang Dianrong. In vitro release of thermosensitive slow-release rectal liquid suppository of insulin[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 56-58,67. doi: 10.3969/j.issn.1006-0111.2014.01.016
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In vitro release of thermosensitive slow-release rectal liquid suppository of insulin

doi: 10.3969/j.issn.1006-0111.2014.01.016
  • 1.

    School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208th Hospital of PLA, Changchun 130021, China

  • 2.

    No. 208th Hospital of PLA, Changchun 130021, China

  • 3.

    School of Pharmacy, Yanbian University, Yanji 133000, China

  • 4.

    The Vet Ranch of Haotetaohai, Inner Mongolia Hailaer 021000, China

  • Received Date: 2013-06-05
  • Rev Recd Date: 2013-11-21
  • Objective To establish HPLC method for the content determination of insulin in thermosensitive slow-release rectal liquid suppository, evaluate release characteristics in vitro. Methods The separation was performed on a Welch ultimate column(4.6 mm×250 mm, 5 μm).The mobile phase was composed of 0.2 mol/L sodium sulfate buffer (pH was adjusted to 2.3 with ethanolamine)-acetonitrile (74:26) with flow rate 1ml/min, detected at 214 nm, the insulin levels were determined. Adopting membrane less release model, bovine intestinal mucosa release model, the gut mucosa release model, insulin was used as model drug, drug release mechanism of thermosensitive slow-release rectal liquid suppository of insulin in vitro was evaluate. Results The insulin in situ gel was linear in the range of 1.0-40.0 U/ml(r=0.999 1).The average recovery was 99.35%, RSD was 0.79;membrane-less release:Q=0.495 4t-2.611 8(r=0.997 7);bovine intestinal mucosa release:(1-Q)1/3=0.001 6t+2.927 4(r=0.975 5);gut-mucosa release:lnQ=0.878 3lnt-4.931 1(r=0.970 5). Conclusion The method was simple, fine repeatability, sensitive, accurate and reliable. The method could be applicable to the preparation of the content determination of insulin and evaluate release mechanism thermosensitive slow-release rectal liquid suppository of insulin.
  • [1] Hincheliffe M, Ilium L. Intranasal insulin delivery and therapy[J]. Adv Drug Devil Rev, 1999, 35:199.
    [2] 何文,代文兵,吴燕,等.N-三甲基壳聚糖对胰岛素液体栓剂直肠吸收的促进作用研究[J].中国药学杂志,2006,4(16):1240.
    [3] 国家药典委员会,中华人民共国药典2010年版二部[S].北京:中国医药科技出版社,2010:846.
    [4] Desai SD,Blanchard J.In vitro evaluation of pluronic F127-based controlled-release ocular delivery systems for pilocarpine[J].J Pham Sci, 1998,87:226.
    [5] 袁园,王晓辉,张莉,等.无膜释放模型考察直肠用温敏型原位凝胶的体外释放情况[J].中国药房,2011,22(29):2750.
    [6] 王军.胰岛素眼用温度敏感即型凝胶的制备及其体外释药考察[J].广东药学院学报,2011,27(4):1132.
    [7] 冯敏,何文.眼用维生素A棕榈酸酯阳离子脂质体原位凝胶的研制及体外释药研究[J].中国药师,,2010,13(5):631.
    [8] 肖金宝,李洪斌,刘福强,等.银杏叶提取物温度敏感型原位凝胶体外释放研究[J].中成药,2013,35(1):46.
    [9] 梁文权.生物药剂学与药物动力学[M].北京:人民卫生出版社,2003:79.
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In vitro release of thermosensitive slow-release rectal liquid suppository of insulin

doi: 10.3969/j.issn.1006-0111.2014.01.016
  • 1. School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208th Hospital of PLA, Changchun 130021, China
  • 2. No. 208th Hospital of PLA, Changchun 130021, China
  • 3. School of Pharmacy, Yanbian University, Yanji 133000, China
  • 4. The Vet Ranch of Haotetaohai, Inner Mongolia Hailaer 021000, China
  • Received Date: 2013-06-05
  • Rev Recd Date: 2013-11-21

Abstract: Objective To establish HPLC method for the content determination of insulin in thermosensitive slow-release rectal liquid suppository, evaluate release characteristics in vitro. Methods The separation was performed on a Welch ultimate column(4.6 mm×250 mm, 5 μm).The mobile phase was composed of 0.2 mol/L sodium sulfate buffer (pH was adjusted to 2.3 with ethanolamine)-acetonitrile (74:26) with flow rate 1ml/min, detected at 214 nm, the insulin levels were determined. Adopting membrane less release model, bovine intestinal mucosa release model, the gut mucosa release model, insulin was used as model drug, drug release mechanism of thermosensitive slow-release rectal liquid suppository of insulin in vitro was evaluate. Results The insulin in situ gel was linear in the range of 1.0-40.0 U/ml(r=0.999 1).The average recovery was 99.35%, RSD was 0.79;membrane-less release:Q=0.495 4t-2.611 8(r=0.997 7);bovine intestinal mucosa release:(1-Q)1/3=0.001 6t+2.927 4(r=0.975 5);gut-mucosa release:lnQ=0.878 3lnt-4.931 1(r=0.970 5). Conclusion The method was simple, fine repeatability, sensitive, accurate and reliable. The method could be applicable to the preparation of the content determination of insulin and evaluate release mechanism thermosensitive slow-release rectal liquid suppository of insulin.

YANG Wenhui, XIAO Qiusheng, JIAG Zongwen, ZHANG Su, SONG Haiyan, Wang Dianrong. In vitro release of thermosensitive slow-release rectal liquid suppository of insulin[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 56-58,67. doi: 10.3969/j.issn.1006-0111.2014.01.016
Citation: YANG Wenhui, XIAO Qiusheng, JIAG Zongwen, ZHANG Su, SONG Haiyan, Wang Dianrong. In vitro release of thermosensitive slow-release rectal liquid suppository of insulin[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 56-58,67. doi: 10.3969/j.issn.1006-0111.2014.01.016
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