Analysis of toxic ingredients of Xiao Chai Hu Tang

ZHAO Bai-yun; LI Yue-yue; ZHU Zhen-yu; ZHAO Liang; GE Meng; ZHANG Guo-qing; CHAI Yi-feng

doi:10.3969/j.issn.1006-0111.2013.06.016

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Objective To determine whether Xiao Chai Hu Tang have liver toxicity and the sources of toxicity. Methods HPLC-TOF/MS method and the MTT were used to assay the Xiao Chai Hu Tang chemical composition and the survival of SMMC-7721 cells, respectively. Then the stepwise multiple linear regression was used to analysis the chemical composition of Xiao Chai Hu Tang which affected SMMC-7721 cells survival. Results The possible toxic component were found from Scutellaria:X3, chrysin-8-C-β-D-glucose the pyrano base-6-C-α in-L-Arab pyran-glucosidase or its isomers. Conclusion The method initially found potentially toxic constituents of Xiao Chai Hu Tang, which provided a research base for Attenuated compatibility, in order to optimize the prescription, develop the preparation process of science, and improve the quality of the preparations.

Analysis of toxic ingredients of Xiao Chai Hu Tang

1. Department of Pharmacy, Hangzhou Normal University Affiliated Hospital, Hangzhou 310000, China

2. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

3. School of Pharmacy, Second Millitary Medical University, Shanghai 200433, China

Received Date: 2012-09-08

Rev Recd Date: 2012-12-08

Key words:

Abstract: Objective To determine whether Xiao Chai Hu Tang have liver toxicity and the sources of toxicity. Methods HPLC-TOF/MS method and the MTT were used to assay the Xiao Chai Hu Tang chemical composition and the survival of SMMC-7721 cells, respectively. Then the stepwise multiple linear regression was used to analysis the chemical composition of Xiao Chai Hu Tang which affected SMMC-7721 cells survival. Results The possible toxic component were found from Scutellaria:X3, chrysin-8-C-β-D-glucose the pyrano base-6-C-α in-L-Arab pyran-glucosidase or its isomers. Conclusion The method initially found potentially toxic constituents of Xiao Chai Hu Tang, which provided a research base for Attenuated compatibility, in order to optimize the prescription, develop the preparation process of science, and improve the quality of the preparations.

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Analysis of toxic ingredients of Xiao Chai Hu Tang

doi: 10.3969/j.issn.1006-0111.2013.06.016

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通讯作者: 陈斌, [email protected]

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