Extraction optimization and content determination of three effective ingredients in Moutan Coretex

ZHAO Liang; WU Hao; ZHANG Hai; LV Lei; ZHU Zhen-yu; ZHANG Guo-qing; CHAI Yi-feng

doi:10.3969/j.issn.1006-0111.2013.06.009

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Objective To optimize the extraction methods and develop a HPLC-DAD assay for the determination of three effective ingredients in Moutan Coretex. Methods The analysis was performed on an Agilent ZORBAX Eclipse Plus (4.6 mm×150 mm, 5 μm) column with acetonitrile and water (0.6% formic acid) as mobile phase at a rate of 1.0 ml/min, the detection wavelength was 254 nm, the injection volume was 10 μl. Results The regress equation of gallic acid, paeoniflorin and paeonol were Y=16.598 0X+4.950 2, r=0.999 9; Y=1.613 1X+0.995 1, r=0.999 9; Y=5.981 7X+54.103 0, r=0.999 7, respectively. The average recoveries were 100.7%, 99.2%, 97.8% and RSD(n=9) were 1.6%, 3.0%, 1.1%. Conclusion The extraction effect was good and the determination assay was reliable and accurate.

Extraction optimization and content determination of three effective ingredients in Moutan Coretex

1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China

2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2012-09-17

Rev Recd Date: 2013-02-21

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Abstract: Objective To optimize the extraction methods and develop a HPLC-DAD assay for the determination of three effective ingredients in Moutan Coretex. Methods The analysis was performed on an Agilent ZORBAX Eclipse Plus (4.6 mm×150 mm, 5 μm) column with acetonitrile and water (0.6% formic acid) as mobile phase at a rate of 1.0 ml/min, the detection wavelength was 254 nm, the injection volume was 10 μl. Results The regress equation of gallic acid, paeoniflorin and paeonol were Y=16.598 0X+4.950 2, r=0.999 9; Y=1.613 1X+0.995 1, r=0.999 9; Y=5.981 7X+54.103 0, r=0.999 7, respectively. The average recoveries were 100.7%, 99.2%, 97.8% and RSD(n=9) were 1.6%, 3.0%, 1.1%. Conclusion The extraction effect was good and the determination assay was reliable and accurate.

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Extraction optimization and content determination of three effective ingredients in Moutan Coretex

doi: 10.3969/j.issn.1006-0111.2013.06.009

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