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Volume 31 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(5): 380-383

LI Huan-yu, DENG Zhao-hui. Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 380-383. doi: 10.3969/j.issn.1006-0111.2013.05.018
Citation: LI Huan-yu, DENG Zhao-hui. Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 380-383. doi: 10.3969/j.issn.1006-0111.2013.05.018
shu

Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC

doi: 10.3969/j.issn.1006-0111.2013.05.018
  • 1.

    Guangzhou Sanatorium of PLA, Guangzhou 510515, China

  • 2.

    Institute for Drug and Instrument Control of Guangzhou Military Area, Guangzhou 510500, China

  • Received Date: 2012-07-27
  • Rev Recd Date: 2013-01-21
  • Objective To establish a method for the simultaneous determination of vitamin C and vitamin B1 in ferrous fumarate granules. Methods The procedure was performed on the Insteril ODS-3 C18 column at 30℃, detected at 244 nm. The mobile phase was composed by a gradient of 0.03 mol/L of ammonium phosphate solution (in which 0.01% of sodium heptanesulfonate was added and the pH was adjusted to 3.0 by phosphoric acid) and methanol, with the flux at 0.6~1.0 ml/min. Results Either vitamin C or vitamin B1 was in good linear at 0.501~6.011 μg, r=1.0000 and 0.038~0.456 μg, r=0.999 9, respectively. The mean recoveries were 99.51% and 99.20%, with the RSD 0.55% and 0.49%, n=9. Conclusion The method was proved to be simple, accurate and reliable, appropriate for the quality control for vitamin C and vitamin B1 in ferrous fumarate granules.
  • [1] 陈新谦,金有豫,汤 光. 新编药物学[M].第17版.北京:人民卫生出版社,2011:778.
    [2] 国家药典委员会.《国家药品标准》化学药品地方标准上升国家标准第三册[S].2002:3.
    [3] 何笑荣,姜文清,霍秀颖,等.RP-HPLC法同时测定利明滴眼液中维生素C和维生素B1的含量[J].中国药房,2012,23(17):1613.
    [4] 张 爽,张 华,赵思奇.维生素C稳定性紫外分光光度测定法的建立[J].黑龙江医药,2008,21(1):26.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC

doi: 10.3969/j.issn.1006-0111.2013.05.018
  • 1. Guangzhou Sanatorium of PLA, Guangzhou 510515, China
  • 2. Institute for Drug and Instrument Control of Guangzhou Military Area, Guangzhou 510500, China
  • Received Date: 2012-07-27
  • Rev Recd Date: 2013-01-21

Abstract: Objective To establish a method for the simultaneous determination of vitamin C and vitamin B1 in ferrous fumarate granules. Methods The procedure was performed on the Insteril ODS-3 C18 column at 30℃, detected at 244 nm. The mobile phase was composed by a gradient of 0.03 mol/L of ammonium phosphate solution (in which 0.01% of sodium heptanesulfonate was added and the pH was adjusted to 3.0 by phosphoric acid) and methanol, with the flux at 0.6~1.0 ml/min. Results Either vitamin C or vitamin B1 was in good linear at 0.501~6.011 μg, r=1.0000 and 0.038~0.456 μg, r=0.999 9, respectively. The mean recoveries were 99.51% and 99.20%, with the RSD 0.55% and 0.49%, n=9. Conclusion The method was proved to be simple, accurate and reliable, appropriate for the quality control for vitamin C and vitamin B1 in ferrous fumarate granules.

LI Huan-yu, DENG Zhao-hui. Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 380-383. doi: 10.3969/j.issn.1006-0111.2013.05.018
Citation: LI Huan-yu, DENG Zhao-hui. Quantitative determination of vitamin C and vitamin B1 in ferrous fumarate granules by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 380-383. doi: 10.3969/j.issn.1006-0111.2013.05.018
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