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Volume 31 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(4): 300-301,317

CHU Zhong-ying, WU Li-hong, XU Hong-yan. Determination of chlorogenic acid and hesperidin in Lishi Oral Liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 300-301,317. doi: 10.3969/j.issn.1006-0111.2013.04.017
Citation: CHU Zhong-ying, WU Li-hong, XU Hong-yan. Determination of chlorogenic acid and hesperidin in Lishi Oral Liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 300-301,317. doi: 10.3969/j.issn.1006-0111.2013.04.017
shu

Determination of chlorogenic acid and hesperidin in Lishi Oral Liquid by HPLC

doi: 10.3969/j.issn.1006-0111.2013.04.017

  • Shanghai Songjiang Institute for Food and Drug Control, Shanghai 201600, China

  • Received Date: 2012-07-18
  • Rev Recd Date: 2012-12-10
  • Objective To establish a method for determining chlorogenic acid and hesheridin in Lishi Oral Liquid (Plantaginis Herba, Kochiate Frouctus, Lonicerae Japonicae Flos, Sophorpe Flavescentis Radix, Citri Reticulatae Pericarpium, Poriae Cutis, Maifahitum) by HPLC. Methods The HPLC with a C18 column was used.The mobile phases was a mixture of acetonitrile for current phase A, 1% Phosphoric acid solution for current phase B; the form prescribed in the gradient elution. The detection wavelength was 290 nm, flow velocity was 1.0 ml/min. Results The linear range was 4.040~80.80 μg/ml for chlorogenic acid and 3.618~72, 35 μg/ml for hesperidin.The average recovery of chlorogenic acid was 100.2% (RSD was 0.83%). The average recovery of hesperidin was 99.95%(RSD was 0.61%). Conclusions The method was simple, accurate, and reproducible with exclusive propertywhich could be used for quality control of Lishi Oral Liquid.
  • [1] 上海市食品药品监督管理局医疗机构制剂质量标准[S],SYZ-ZF-003-2004.
    [2] 黄丽瑛,吕植桢,李继彪,等.中药金银花化学成分的研究[J].中草药,1996,15(11):645.
    [3] 张志海,王彩云,杨天鸣,等.陈皮的化学成分及药理作用研究进展[J].西北药学杂志, 2005,20(1):49.
    [4] 中国药典2010年版[S].2010:205,176,1089,1077,1139.
    [5] 邢丽红,李文龙,瞿海斌. 金银花提取物中5种有机酸含量测定的紫外光谱法[J].药物分析杂志,2011,31(3):133.
    [6] 杨蓓蓓,刘超,王素娟,等.高效液相色谱法测定金银花药材中3种成分的含量[J].药物分析杂志. 2006,26(2):34.
    [7] 林林,林子夏,莫云燕,等. 不同年份新会陈皮总黄酮及橙皮苷含量动态分析[J]. 时珍国医国药.2008,19(6):150.
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Determination of chlorogenic acid and hesperidin in Lishi Oral Liquid by HPLC

doi: 10.3969/j.issn.1006-0111.2013.04.017
  • Shanghai Songjiang Institute for Food and Drug Control, Shanghai 201600, China
  • Received Date: 2012-07-18
  • Rev Recd Date: 2012-12-10

Abstract: Objective To establish a method for determining chlorogenic acid and hesheridin in Lishi Oral Liquid (Plantaginis Herba, Kochiate Frouctus, Lonicerae Japonicae Flos, Sophorpe Flavescentis Radix, Citri Reticulatae Pericarpium, Poriae Cutis, Maifahitum) by HPLC. Methods The HPLC with a C18 column was used.The mobile phases was a mixture of acetonitrile for current phase A, 1% Phosphoric acid solution for current phase B; the form prescribed in the gradient elution. The detection wavelength was 290 nm, flow velocity was 1.0 ml/min. Results The linear range was 4.040~80.80 μg/ml for chlorogenic acid and 3.618~72, 35 μg/ml for hesperidin.The average recovery of chlorogenic acid was 100.2% (RSD was 0.83%). The average recovery of hesperidin was 99.95%(RSD was 0.61%). Conclusions The method was simple, accurate, and reproducible with exclusive propertywhich could be used for quality control of Lishi Oral Liquid.

CHU Zhong-ying, WU Li-hong, XU Hong-yan. Determination of chlorogenic acid and hesperidin in Lishi Oral Liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 300-301,317. doi: 10.3969/j.issn.1006-0111.2013.04.017
Citation: CHU Zhong-ying, WU Li-hong, XU Hong-yan. Determination of chlorogenic acid and hesperidin in Lishi Oral Liquid by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 300-301,317. doi: 10.3969/j.issn.1006-0111.2013.04.017
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