WANG Tian-qi, LI Xiang, LI Ling, WU Hai-tang, XU Li. Determination of sterols in Candida albicans by GC-SIM-MS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 277-279,320. doi: 10.3969/j.issn.1006-0111.2013.04.010
| Citation:
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WANG Tian-qi, LI Xiang, LI Ling, WU Hai-tang, XU Li. Determination of sterols in Candida albicans by GC-SIM-MS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 277-279,320. doi: 10.3969/j.issn.1006-0111.2013.04.010
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Determination of sterols in Candida albicans by GC-SIM-MS
- 1.
School of Pharmacy, Second Military Medical University, Shanghai 200433 China
- 2.
No. 62380 PLA troops, Beijing 100071 China
- 3.
Department of Medicinal Instrument, The 113th Hospital of PLA, Ningbo 315040 China
- Received Date: 2012-12-04
- Rev Recd Date:
2013-02-28
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Abstract
Objective To establish a method of GC-SIM-MS for the determination of sterols in Candida albicans, including lanosterol and ergosterol. Methods The sterols extracted from Candida albicans were analyzed by gas chromatography with a HP 50 capillary column, temperature from 150℃ to 300℃, 10℃/min, then, increased from 300℃ to 320℃, 20℃/min;detected temperature was 270℃; injection temperature was 270℃; carrier gas was Helium, flow rate was 1 ml/min; no splic.Mass spectrometry:EI Ion source, Ionization voltage:70 eV; SIM Scan; the selected ions:3.00~17.50 min, 275, 386; 17.50~19.00min, 363, 396;19.00~30.00 min 411, 426. Results Linear relation of lanosterol and ergosterol were good(r=0.999 8 and 0.999 5 respectively). The average recoveries were between 91.2%~117.3%, RSD<2.5%. The lowest limit of detect was 18.29 ng/ml and 68.13 ng/ml respectively, the precision within day were good (RSD<1.2%). Conclusion This method was accurate, sensitive and specific. The results were reliable, which provided a powerful tool for the study of antifungal agents.
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