Effect of icariin and curculigoside on inhibition of osteoclastic formation, differentiation and bone absorption

GUO Yuan-hui; XUE Li-ming; NIE Yan; WANG Ying; ZHAO Lu; QIN Lu-Ping; ZHANG Qiao-yan; YU Yuan

doi:10.3969/j.issn.1006-0111.2013.04.006

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Objective To investigate the interaction relationship of icariin and curculigoside on the osteoclastic formation, differentiation and bone resorbing. Methods Multinucleated osteoclasts were induced by 1, 25-dihydroxyvitamine D₃ and dexamethasone from bone marrow cells with the presence of primary osteoblastic cells, or induced with RANKL and MCSF.The activity of TRAP was measured by p-nitrophenyl sodium phosphate assay.The bone absorption pit area on the bone slices formed by osteoclast was measured by computer image processing.The F-actin ring were stained with Hoechst 33 258 and rhodamine-phalloidin, and then observed under the confocal scanning laser microscopy. The Western-blot was used to analysis the expression of protein which was related to the cytoskeleton of osteoclasts. Results Icariin and curculigoside at the concentration of 1×10^-6mol/L synergistically inhibited the osteoclastic formation, decreased the activity of TRAP, and reduced the area of bone resorption pit on bone slices, inhibited the organization of F-actin ring and the expression of FAK and Rho GTPases. Conclusions Icariin and curculigoside synergistically inhibited the osteoclastic bone resorption, which provided scientific basis for the theory of the compatibility of traditional Chinese medicine Epimedii Folium and CurculiginIs Rhizoma.

Effect of icariin and curculigoside on inhibition of osteoclastic formation, differentiation and bone absorption

1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China

Received Date: 2012-03-22

Rev Recd Date: 2012-04-25

Key words:

Abstract: Objective To investigate the interaction relationship of icariin and curculigoside on the osteoclastic formation, differentiation and bone resorbing. Methods Multinucleated osteoclasts were induced by 1, 25-dihydroxyvitamine D₃ and dexamethasone from bone marrow cells with the presence of primary osteoblastic cells, or induced with RANKL and MCSF.The activity of TRAP was measured by p-nitrophenyl sodium phosphate assay.The bone absorption pit area on the bone slices formed by osteoclast was measured by computer image processing.The F-actin ring were stained with Hoechst 33 258 and rhodamine-phalloidin, and then observed under the confocal scanning laser microscopy. The Western-blot was used to analysis the expression of protein which was related to the cytoskeleton of osteoclasts. Results Icariin and curculigoside at the concentration of 1×10^-6mol/L synergistically inhibited the osteoclastic formation, decreased the activity of TRAP, and reduced the area of bone resorption pit on bone slices, inhibited the organization of F-actin ring and the expression of FAK and Rho GTPases. Conclusions Icariin and curculigoside synergistically inhibited the osteoclastic bone resorption, which provided scientific basis for the theory of the compatibility of traditional Chinese medicine Epimedii Folium and CurculiginIs Rhizoma.

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Reference (14)

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Effect of icariin and curculigoside on inhibition of osteoclastic formation, differentiation and bone absorption

doi: 10.3969/j.issn.1006-0111.2013.04.006

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通讯作者: 陈斌, [email protected]

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