Determination of hydroxysaffor yellow A in Yihe decoction by HPLC

SHA Ri-na; HE Chun-long; WANG Huan-yun

doi:10.3969/j.issn.1006-0111.2013.02.017

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Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C₁₈ column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction.

Determination of hydroxysaffor yellow A in Yihe decoction by HPLC

1. Inner Mongolia Medical university, Department of pharmacy, Second affiliated hospita

2. School of Pharmay, Hohhot 010030, China

Received Date: 2012-06-25

Rev Recd Date: 2012-09-19

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Abstract: Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C₁₈ column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction.

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Determination of hydroxysaffor yellow A in Yihe decoction by HPLC

doi: 10.3969/j.issn.1006-0111.2013.02.017

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通讯作者: 陈斌, [email protected]

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