SHI Hong. Determination of ursolic acid and oleanolic acid in Salvia chinensis Benth by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 134-136. doi: 10.3969/j.issn.1006-0111.2013.02.015
| Citation:
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SHI Hong. Determination of ursolic acid and oleanolic acid in Salvia chinensis Benth by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 134-136. doi: 10.3969/j.issn.1006-0111.2013.02.015
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Determination of ursolic acid and oleanolic acid in Salvia chinensis Benth by HPLC
- Received Date: 2012-11-23
- Rev Recd Date:
2013-02-05
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Abstract
Objective To establish a method for determination of ursolie acid and oleanolic acid contents in Salvia chinensis Benth. Methods HPLC-DAD was used;the column was Agilent HC18 (4.6 mm×250 mm,5μm);mobile phase was A:0.1%(v/v)formic acid solution,B:methanol,A:B=14:86. The flow rate was 1.0 ml/min;the temperature of column was 20℃;the detection wavelength was set at 205 nm;the injection volume was 50 μl; the running time was 35 min. Results Ursolic acid and oleanolic acid were separated in baseline, the linear range was 11.55~462.0 μg/ml for ursolic acid and 5.265~210.6 μg/ml for oleanolic acid. The result of intra-day and inter-day precisions were both within 5%(n=3), and the average recovery was 100.47%(RSD=1.95%, n=6)and 101.88%(RSD=2.98%, n=6). Conclusion The method was rapid,convenient,accurate,and suitable for quality control of ursolic and oleanolic acid in Salvia chinensis Benth.
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