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Volume 31 Issue 2
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(2): 116-119

ZHOU Yong-gang, WU Si, MAO Fei, WU Ting-ting, WU Hai-tang, CHAI Yi-feng. Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 116-119. doi: 10.3969/j.issn.1006-0111.2013.02.009
Citation: ZHOU Yong-gang, WU Si, MAO Fei, WU Ting-ting, WU Hai-tang, CHAI Yi-feng. Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 116-119. doi: 10.3969/j.issn.1006-0111.2013.02.009
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Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS

doi: 10.3969/j.issn.1006-0111.2013.02.009
  • 1.

    The 81st Hospital of PLA, Nanjing 210002, China

  • 2.

    School of Pharmacy, Second Military Medical University, Shanghai 200433 China

  • 3.

    Department of Pharmacy, Beijing Children's Hospital, Beijing 100056, China

  • 4.

    Department of Drug and Equipment Section, The 263rd Hospital of PLA, Beijing 101149, China

  • Received Date: 2012-08-15
  • Rev Recd Date: 2012-10-29
  • Objective To analyze chemical constituents of Pueraria lobata by ultrahigh pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOFMS). Methods A ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.4 ml/min. TOFMS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 19 major constituents in Gardenia jasminoides Ellis were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using UHPLC-Q-TOFMS was established to identify the chemical constituents of Pueraria lobata.
  • [1] 柳 航, 李占林, 郭 婕, 等.葛根的化学成分[J].沈阳药科大学学报,2009, 26:882.
    [2] Du G, Zhao HY, Zhang QW, et al, A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry[J]. J Chromatogr A, 2010, 1217:705.
    [3] 石立秋.葛根的化学成分及药理活性[J].中华临床医学研究杂志, 2003,70:11678.
    [4] 董 英, 徐 斌, 林 琳, 等. 葛根的化学成分研究[J]. 食品与机械, 2005,21(6):85.
    [5] 杨东晖, 陈 浪, 曾 志, 等.葛根化学成分的研究[J]. 华南师范大学学报, 2002,94:94.
    [6] 田宏哲, 王 华, 关亚风.液质联用分析葛根提取物及中药片剂中异黄酮类化合物[J]. 色谱, 2005, 23(5):477.
    [7] Lin CC, Wu CI, Shen SJ, Determination of 12 pueraria components by high-performance liquid chromatography-mass spectrometry[J]. J Sep Sci, 2005, 28:1785.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS

doi: 10.3969/j.issn.1006-0111.2013.02.009
  • 1. The 81st Hospital of PLA, Nanjing 210002, China
  • 2. School of Pharmacy, Second Military Medical University, Shanghai 200433 China
  • 3. Department of Pharmacy, Beijing Children's Hospital, Beijing 100056, China
  • 4. Department of Drug and Equipment Section, The 263rd Hospital of PLA, Beijing 101149, China
  • Received Date: 2012-08-15
  • Rev Recd Date: 2012-10-29

Abstract: Objective To analyze chemical constituents of Pueraria lobata by ultrahigh pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOFMS). Methods A ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.4 ml/min. TOFMS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 19 major constituents in Gardenia jasminoides Ellis were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using UHPLC-Q-TOFMS was established to identify the chemical constituents of Pueraria lobata.

ZHOU Yong-gang, WU Si, MAO Fei, WU Ting-ting, WU Hai-tang, CHAI Yi-feng. Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 116-119. doi: 10.3969/j.issn.1006-0111.2013.02.009
Citation: ZHOU Yong-gang, WU Si, MAO Fei, WU Ting-ting, WU Hai-tang, CHAI Yi-feng. Analysis on flavonoid constituents of Pueraria lobata by UHPLC-Q-TOFMS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 116-119. doi: 10.3969/j.issn.1006-0111.2013.02.009
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