Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 31 Issue 2
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(2): 113-115

HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
Citation: HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
shu

Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD

doi: 10.3969/j.issn.1006-0111.2013.02.008
  • 1.

    Changhai Hospital, Second Military Medical University, Department of Nursing

  • 2.

    Department of Pharmacy, Shanghai 200433, China

  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-07-24
  • Objective To establish a HPLC-ECD method for determination of dopamine hydrochloride in prefilled syringe. Methods The determination was performed on a Diamonsil-C18 analytical column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-0.11% sodium heptanesulfonate solution (containing 0.1 mmol/L EDTA-2Na and 5% glacial acetic acid, adjusting to pH 3.4 with 1 mol/L NaOH). The silver/silver chloride electrode was used as reference electrode and the glassy carbon electrode was used as the working electrode at the potential of+0. 65 V.The flow rate was 1.0 ml/min and column temperature was 20℃. Results The calibration curve of dopamine hydrochloride were linear in the concentration range of 0.05~5 μg/ml (r=0.999 9,n=5). The average recovery rate of dopamine hydrochloride was (99.19±4.03)% (n=3). RSD of intra-day and inter-day were less than 4% (n=3). The contents of dopamine hydrochlorid in three batches of samples were(102.97±1.11)%. Conclusion The method was accurate,rapid and high sensitivity,which was suitable for the determination of dopamine hydrochloride in prefilled syringe.
  • [1] 潘自红,贺国旭,胡小明,等. 分光光度法测定盐酸多巴胺的含量[J]. 分析试验室,2011,30(11):89.
    [2] Nishiyama J, Suzuki T, Murata T, et al. A questionnaire survey on operability of syringe pumps for prefilled syringes[J]. Tokai J Exp Clin Med, 2010, 35(3):95.
    [3] 中国药典2010版.二部[S].2010:688.
    [4] 吴燕琼,梁洁雯. HPLC测定盐酸多巴胺注射液的含量[J].广东药学,2005,15(3):15.
    [5] 宋才勇,余 莉,李雪艳,等. 微透析活体取样与高效液相色谱-电化学检测法联用测定帕金森大鼠脑中神经递质[J]. 分析试验室,2011,30(8):80.
  • 加载中
通讯作者: 陈斌, [email protected]
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2513) PDF downloads(343) Cited by()

Related
Proportional views

Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD

doi: 10.3969/j.issn.1006-0111.2013.02.008
  • 1. Changhai Hospital, Second Military Medical University, Department of Nursing
  • 2. Department of Pharmacy, Shanghai 200433, China
  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-07-24

Abstract: Objective To establish a HPLC-ECD method for determination of dopamine hydrochloride in prefilled syringe. Methods The determination was performed on a Diamonsil-C18 analytical column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-0.11% sodium heptanesulfonate solution (containing 0.1 mmol/L EDTA-2Na and 5% glacial acetic acid, adjusting to pH 3.4 with 1 mol/L NaOH). The silver/silver chloride electrode was used as reference electrode and the glassy carbon electrode was used as the working electrode at the potential of+0. 65 V.The flow rate was 1.0 ml/min and column temperature was 20℃. Results The calibration curve of dopamine hydrochloride were linear in the concentration range of 0.05~5 μg/ml (r=0.999 9,n=5). The average recovery rate of dopamine hydrochloride was (99.19±4.03)% (n=3). RSD of intra-day and inter-day were less than 4% (n=3). The contents of dopamine hydrochlorid in three batches of samples were(102.97±1.11)%. Conclusion The method was accurate,rapid and high sensitivity,which was suitable for the determination of dopamine hydrochloride in prefilled syringe.

HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
Citation: HUANG Juan, ZHU Quan-gang, ZHANG Ling-juan, CAO Jie. Determination of dopamine hydrochloride in prefilled syringe by HPLC-ECD[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(2): 113-115. doi: 10.3969/j.issn.1006-0111.2013.02.008
shu

    HTML

Reference (5)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: [email protected]

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return