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Volume 31 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(1): 61-63

YANG Xiao-ying, MA Qi-zhen. Determination of Esomeprazole in dog plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 61-63. doi: 10.3969/j.issn.1006-0111.2013.01.018
Citation: YANG Xiao-ying, MA Qi-zhen. Determination of Esomeprazole in dog plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 61-63. doi: 10.3969/j.issn.1006-0111.2013.01.018
shu

Determination of Esomeprazole in dog plasma by LC-MS-MS

doi: 10.3969/j.issn.1006-0111.2013.01.018
  • 1.

    General Hospital of Ningxia Medical University, Department of Pharmacy, Yinchuan 750004, China;Division of Clinical Pharmacology, Yinchuan 750004, China

  • 2.

    General Hospital of Ningxia Medical University, Department of Pharmacy, Yinchuan 750004, China

  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-09-28
  • Objective To establish a method for determining Esomeprazole in dog plasma by LC-MS-MS. Methods Diazepam was used as the internal standard,the samples were precipitated with acetonitril. Analytical column was Shim-pack XR-ODS (2 mm×100 mm, 5 μm). The mobile phase was acetonitrile:2 mmol/L ammonium acetate (containing 0.1% formic acid) at a flow rate of 0.3 ml/min. Mass spectrum conditions was ESI performing in positive ion MRM mode using target ions 346.2→198.2 m/z (esomeprazole)and 285.2→193.2 m/z (diazepam). Results The calibration curve was linear over the range of 0.5~1 200 μg/L. The LLOQ of Esomeprazole in plasma was 0.5 μg/L. The average extracted recovery was (101.0±6.5)%. The RSDs of intra- and inter-day were less than 15%. Conclusion The method was sensitive, simple and accurate to determinate Esomeprazole plasma concentration and suitable to the pharmacokinetics study.
  • [1] 杨 丽,严宝霞.埃索美拉唑的药动学[J].中国新药杂志,2003,13(5):398.
    [2] 戴歌心,张振南,王贤亲,等.HPLC-MS 联用测定人血浆中埃索美拉唑的浓度[J].中国卫生检验杂志,2011:21(8):1875.
    [3] 余 勤,梁茂植,赵 焱,等.反相高效液相色谱法测定血浆中埃索美拉唑浓度[J].华西医学,2006:21(1):93.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Determination of Esomeprazole in dog plasma by LC-MS-MS

doi: 10.3969/j.issn.1006-0111.2013.01.018
  • 1. General Hospital of Ningxia Medical University, Department of Pharmacy, Yinchuan 750004, China;Division of Clinical Pharmacology, Yinchuan 750004, China
  • 2. General Hospital of Ningxia Medical University, Department of Pharmacy, Yinchuan 750004, China
  • Received Date: 2012-05-08
  • Rev Recd Date: 2012-09-28

Abstract: Objective To establish a method for determining Esomeprazole in dog plasma by LC-MS-MS. Methods Diazepam was used as the internal standard,the samples were precipitated with acetonitril. Analytical column was Shim-pack XR-ODS (2 mm×100 mm, 5 μm). The mobile phase was acetonitrile:2 mmol/L ammonium acetate (containing 0.1% formic acid) at a flow rate of 0.3 ml/min. Mass spectrum conditions was ESI performing in positive ion MRM mode using target ions 346.2→198.2 m/z (esomeprazole)and 285.2→193.2 m/z (diazepam). Results The calibration curve was linear over the range of 0.5~1 200 μg/L. The LLOQ of Esomeprazole in plasma was 0.5 μg/L. The average extracted recovery was (101.0±6.5)%. The RSDs of intra- and inter-day were less than 15%. Conclusion The method was sensitive, simple and accurate to determinate Esomeprazole plasma concentration and suitable to the pharmacokinetics study.

YANG Xiao-ying, MA Qi-zhen. Determination of Esomeprazole in dog plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 61-63. doi: 10.3969/j.issn.1006-0111.2013.01.018
Citation: YANG Xiao-ying, MA Qi-zhen. Determination of Esomeprazole in dog plasma by LC-MS-MS[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(1): 61-63. doi: 10.3969/j.issn.1006-0111.2013.01.018
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