Elucidation of 10-hydroxy-7-methyl-homocamptothecin by electrospray ion trap mass spectrometry

CUI Xiao-ru; GUAN Xiao-duo; MIAO Zhen-yuan; ZHANG Wan-nian; FAN Guo-rong

doi:10.3969/j.issn.1006-0111.2013.01.007

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Objective To analyze the fragments of 10-hydroxy-7-methyl- homocamptothecin (TOP-01) by electrospray ion trap mass spectrometry and deduce the MS/MS fragment pathway of it. Methods Samples were directly injected into the instrument and the fragments of the samples were yielded by using multi-stage ion trap mass spectrometry. Results Fragment ions m/z 333, 305, 277, 290 were generated at the positive ion mode and m/z 347, 319, 317, 289, 301 at the negative mode for TOP-01. Conclusion Differences existed in fragmentation pathway between positive and negative ESI-MS,and the main fragmentation characteristics of TOP-01 were m/z 333 and 347 in positive and negative mode respectively. These results provided very important information for the structural modification and the drug metabolic research of homocamptothecin.

Elucidation of 10-hydroxy-7-methyl-homocamptothecin by electrospray ion trap mass spectrometry

1. Department of Pharmaceutical Analysis, Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University

2. Department of Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2011-04-09

Rev Recd Date: 2012-06-30

Key words:

homocamptothecin derivatives /
10-hydroxy-7-methyl-homocamptothecin /
electrosprayion trap mass spectrometry /
structure elucidation

Abstract: Objective To analyze the fragments of 10-hydroxy-7-methyl- homocamptothecin (TOP-01) by electrospray ion trap mass spectrometry and deduce the MS/MS fragment pathway of it. Methods Samples were directly injected into the instrument and the fragments of the samples were yielded by using multi-stage ion trap mass spectrometry. Results Fragment ions m/z 333, 305, 277, 290 were generated at the positive ion mode and m/z 347, 319, 317, 289, 301 at the negative mode for TOP-01. Conclusion Differences existed in fragmentation pathway between positive and negative ESI-MS,and the main fragmentation characteristics of TOP-01 were m/z 333 and 347 in positive and negative mode respectively. These results provided very important information for the structural modification and the drug metabolic research of homocamptothecin.

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Reference (8)

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Elucidation of 10-hydroxy-7-methyl-homocamptothecin by electrospray ion trap mass spectrometry

doi: 10.3969/j.issn.1006-0111.2013.01.007

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