Determinatin of chlorogenic acid in hawthorn by HPLC

LIANG Rui-shu; ZHANG Chuan; ZOU Hao; JIN Lei

doi:10.3969/j.issn.1006-0111.2012.06.018

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C₁₈ (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.

Determinatin of chlorogenic acid in hawthorn by HPLC

1. Guang Dong Shuang Lin Bio-pharmacy Company Limited, Zhanjiang 524009, China

2. New Drug Research Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2012-06-01

Rev Recd Date: 2012-09-12

Key words:

chlorogenic acid /
HPLC /
hawthorn

Abstract: Objective To establish a HPLC method for determination of chlorogenic acid in the hawthorn. Methods Sunfire C₁₈ (4.6 mm×250 mm, 5 μm) column was used. The mobile phase was methanol:sodium dihydrogen phosphate(25:75). The flow rate was 1.0 ml/min, and the temperature was at 28℃. The detection wavelength was 327 nm. Results The calibration curve was at the range of 7.03~70.3 μg/ml (r=0.999 6), RSD was 0. 92% (n=6) and the average recovery was 96.92%. Conclusion The method was simple, reliable, accurate for determination of chlorogenic acid in the hawthorn.

HTML

Reference (9)

[1]	郑虎占, 董泽宏, 佘靖. 中药现代研究与应用:第一卷[M]. 北京:学苑出版社, 1997:597.
[2]	冯凤莲, 张卉朱. 山楂的研究进展[J].河北医科大学学报, 1997, 18(6):383.
[3]	高光跃,冯毓秀,秦秀琴.山楂类果实的化学成分分析及其质量评价[J].药学学报,1995,30(2):138.
[4]	袁华,邓良,孙炎彬,等.可见分光光度法测定杜仲叶中的绿原酸[J].生物加工过程,2007,5(1):71.
[5]	朱玉,张书胜,张西林,等.薄层色谱法分析葵花仁粕中的绿原酸[J].色谱,2001,19(1):82.
[6]	张风云,毛富春.用毛细管区带电泳技术测定杜仲叶中绿原酸含量的研究[J].分析测试学报,1996,15(3):66.
[7]	崔伟伟,范永春.不同提取方法测定野马追药材中绿原酸含量比较研究[J].辽宁中医药大学学报,2011,13(3):178.
[8]	韩哲,崔同, 锁然, 等. 山楂果实中活性成分的高效液相色谱法[J].食品科技, 2006(6):124.
[9]	胡志国,王远兴. 反相高效液相色谱法测定山楂中绿原酸的含量[J].食品科学,2007(11):407.

Message Board

Determinatin of chlorogenic acid in hawthorn by HPLC

doi: 10.3969/j.issn.1006-0111.2012.06.018

Abstract

References

Proportional views

通讯作者: 陈斌, [email protected]

Article Metrics

Related

Proportional views