JI Song-gang, LIU Yue, MAO Fei, LI Xiang, CHAI Yi-feng. Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 437-439,472. doi: 10.3969/j.issn.1006-0111.2012.06.011
| Citation:
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JI Song-gang, LIU Yue, MAO Fei, LI Xiang, CHAI Yi-feng. Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 437-439,472. doi: 10.3969/j.issn.1006-0111.2012.06.011
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Analysis on chemical constituents of Scutellaria barbata by HPLC-TOF/MS
- 1.
Department of Pharmacy, the 401st Hospital of PLA, Qingdao 266071, China
- 2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 3.
Department of Pharmacy, Beijing children's hospital, Beijing 100056, China
- Received Date: 2012-09-05
- Rev Recd Date:
2012-09-27
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Abstract
Objective To analyze chemical constituents of Scutellaria barbata D.Don by high pressure liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A SHISEIDO CAPCELL PAK C18 (100 mm×3.0 mm, 3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 15 major constituents in Scutellaria barbata D.Don were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of Scutellaria barbata D.Don.
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