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Volume 30 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(5): 351-352,386

LIAO Li-na, ZHANG Ming-min, CAO Wei-wei, MAO Jun-qin. Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 351-352,386. doi: 10.3969/j.issn.1006-0111.2012.05.009
Citation: LIAO Li-na, ZHANG Ming-min, CAO Wei-wei, MAO Jun-qin. Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 351-352,386. doi: 10.3969/j.issn.1006-0111.2012.05.009
shu

Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)

doi: 10.3969/j.issn.1006-0111.2012.05.009
  • 1.

    Anhui Institute of Traditional Chinese Medicine, Hefei 230038, China

  • 2.

    411st hospital of PLA, Shanghai 200433, China

  • 3.

    85th hospital of PLA, Shanghai 200053, China

  • Received Date: 2012-03-04
  • Rev Recd Date: 2012-05-09
  • To establish a HPLC fingerprint method for Semen Sojae Preparatumto quality control. Methods HPLC analysis was performed on a Diamonsil C18 column (250 mm×4.6 mm,5 μm),the mobile phase was used in gradient elution consisted of methanol-water(3% acetic acid),the flow rate was 1.0 ml /min, column temperature was 30℃ and UV detected was set at 261 nm,the sample weight:10 μl. Results The compound composition of different SSP origins was obviously comparable, which could be determined by HPLC fingerprint of the SSP. Conclusion This method could be utilized as a quality control measurement for SSP.
  • [1] 中国药典2010版.一部[S].2010:625.
    [2] 李娜,黄庆柏.淡豆豉中的异黄酮成分及药理作用与临床作用[J].中国现代中药,2008,10(7):18.
    [3] 陈玉胜, 张李阳. 大豆异黄酮的药理功效研究进展[J].四川生理科学杂志,2011,33(1):26.
    [4] 袁珊亲,于能江,赵毅民,等.淡豆豉中的化学成分[J].中药材,2008,312(8):1172.
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Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)

doi: 10.3969/j.issn.1006-0111.2012.05.009
  • 1. Anhui Institute of Traditional Chinese Medicine, Hefei 230038, China
  • 2. 411st hospital of PLA, Shanghai 200433, China
  • 3. 85th hospital of PLA, Shanghai 200053, China
  • Received Date: 2012-03-04
  • Rev Recd Date: 2012-05-09

Abstract: To establish a HPLC fingerprint method for Semen Sojae Preparatumto quality control. Methods HPLC analysis was performed on a Diamonsil C18 column (250 mm×4.6 mm,5 μm),the mobile phase was used in gradient elution consisted of methanol-water(3% acetic acid),the flow rate was 1.0 ml /min, column temperature was 30℃ and UV detected was set at 261 nm,the sample weight:10 μl. Results The compound composition of different SSP origins was obviously comparable, which could be determined by HPLC fingerprint of the SSP. Conclusion This method could be utilized as a quality control measurement for SSP.

LIAO Li-na, ZHANG Ming-min, CAO Wei-wei, MAO Jun-qin. Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 351-352,386. doi: 10.3969/j.issn.1006-0111.2012.05.009
Citation: LIAO Li-na, ZHANG Ming-min, CAO Wei-wei, MAO Jun-qin. Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 351-352,386. doi: 10.3969/j.issn.1006-0111.2012.05.009
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