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Volume 30 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(3): 226-228

ZHAO Nan, LIU Dan, WANG Yan-ping, WANG Jin-feng, WEI Ying. Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 226-228. doi: 10.3969/j.issn.1006-0111.2012.03.020
Citation: ZHAO Nan, LIU Dan, WANG Yan-ping, WANG Jin-feng, WEI Ying. Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 226-228. doi: 10.3969/j.issn.1006-0111.2012.03.020
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Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.020

  • The No.208 Hospital of PLA,Changchun 130062,China

  • Received Date: 2012-01-10
  • Rev Recd Date: 2012-04-06
  • Objective To establish an HPLC method for the determination of Tectoridin and Tectorigenin in Flos Puerariae lobata. Method The separation was performed on Agilent -C18 column(4.6 mm×250 mm,5 μm),using a mobile phase with methanol-acetonitrile-water(2∶1∶2). Flow rate was 1.0ml/min,the detection wavelength was set at 264 nm. Column temperature was ambient temperature. Result The linear range of tectoridin was 2.0 ~120.0 μg/ml,r=0.999 8.The average recovery was 99.7%,RSD was 1.9%;The linear range of tectorigenin was 0.8 ~80.0 μg/ml,r=0.998 6.The average recovery was 99.4%,RSD was 1.7%. Conclusion The method was simple ,accurate,reliable with a good repeatability,which could be used to determinate of tectoridin and tectorigenin in Flos Puerariae lobata.
  • [1] 田代华.实用中药辞典.下卷[M].北京.人民卫生出版社,2002:1338,1897.
    [2] 尹俊亭,仲 英,孙敬勇,等.葛花化学成分的研究[J].中草药,2006,37(3):350.
    [3] 张淑萍,贺 云,刘柏涛,等.RP-HPLC测定野葛花中3种异黄酮[J].分析实验室,2006,25(2):74.
    [4] 张国峰,吴 瑕,白 雪,等.HPLC法同时测定野葛花中4种异黄酮成分的含量[J].沈阳药科大学学报,2009,26(1):40.
    [5] 刘立辉,李卫民,高 英. HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖糖苷、鸢尾苷、鸢尾苷元的含量[J].中国中药杂志,2010,35(17):2308.
    [6] Kim CS,Shin SM,Ha HK,et al. Study of substance change in flowers of Pueraria thunbergiana B enth. during storage[J].Arch Pharm Res,2003,26(3):210.
    [7] Niiho Y, Nakajima Y, Yamazaki T, et al. Simultaneous analysis of isoflavones and saponins in Pueraria flowers using hplc coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside[J]. J Nat Med, 2010,64(3):313.
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Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.020
  • The No.208 Hospital of PLA,Changchun 130062,China
  • Received Date: 2012-01-10
  • Rev Recd Date: 2012-04-06

Abstract: Objective To establish an HPLC method for the determination of Tectoridin and Tectorigenin in Flos Puerariae lobata. Method The separation was performed on Agilent -C18 column(4.6 mm×250 mm,5 μm),using a mobile phase with methanol-acetonitrile-water(2∶1∶2). Flow rate was 1.0ml/min,the detection wavelength was set at 264 nm. Column temperature was ambient temperature. Result The linear range of tectoridin was 2.0 ~120.0 μg/ml,r=0.999 8.The average recovery was 99.7%,RSD was 1.9%;The linear range of tectorigenin was 0.8 ~80.0 μg/ml,r=0.998 6.The average recovery was 99.4%,RSD was 1.7%. Conclusion The method was simple ,accurate,reliable with a good repeatability,which could be used to determinate of tectoridin and tectorigenin in Flos Puerariae lobata.

ZHAO Nan, LIU Dan, WANG Yan-ping, WANG Jin-feng, WEI Ying. Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 226-228. doi: 10.3969/j.issn.1006-0111.2012.03.020
Citation: ZHAO Nan, LIU Dan, WANG Yan-ping, WANG Jin-feng, WEI Ying. Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(3): 226-228. doi: 10.3969/j.issn.1006-0111.2012.03.020
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