Determination of fluoxetine hydrochloride in plasma by HPLC

XU Xue-jun; TAO Qing; XU De-qin

doi:10.3969/j.issn.1006-0111.2012.03.019

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acetonitrile. Separation was performed on a Hypersil ODS2 column(5 μm, 4.6 mm×200 mm). The mobile phase contained acetonitrile-25 mmol/ L phosphate buffer(pH6.5)(34:66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0.5~50 ng/ml and coefficient was 0.996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7%,97.06% and 100.75%, respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.

Determination of fluoxetine hydrochloride in plasma by HPLC

1. Department of Pharmacy, Anhui General Hospital of the Chinese People's Armed Police Forces, Hefei 230061, China

2. Department of Pharmacy ,The 108th Hospital of PLA, Shanghai 200336,China

Received Date: 2012-02-10

Rev Recd Date: 2012-03-27

Key words:

Abstract: Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acetonitrile. Separation was performed on a Hypersil ODS2 column(5 μm, 4.6 mm×200 mm). The mobile phase contained acetonitrile-25 mmol/ L phosphate buffer(pH6.5)(34:66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0.5~50 ng/ml and coefficient was 0.996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7%,97.06% and 100.75%, respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.

HTML

Reference (10)

[1]	Cheer SM, Goa KL. Fluoxetine: a review of its therapeutic potential in the treatment of depression associated with physical illness[J]. Drugs, 2001, 61(1)∶81.
[2]	汪春运,臧家平. 氟西汀的临床应用[J]. 医药导报,2005,24(10):897.
[3]	刘伟忠,陈清霞,黄伟侨,等. 高效液相色谱法同时测定人血浆中氯氮平与氟西汀的浓度[J]. 广东药学院学报,2011,27(3):243.
[4]	李迎春,姚国灿,郭兴杰,等. 高效液相色谱法测定大鼠脑微渗析液中氟西汀的浓度[J]. 中国药房,2006,17(5):341.
[5]	何娟,周志凌,李焕德. HPLC-MS同时测定4种新型抗抑郁药物的血药浓度[J]. 药物分析杂志,2005,25(12):1428.
[6]	贾晶莹, 张梦琪, 桂雨舟, 等. 液相色谱-串联质谱法同时测定7种抗抑郁类和5种抗精神病类药物的血药浓度[J]. 中国药物应用与监测,2010,7(5):272.
[7]	Franceschi L, Faggiani A, Furlanut M. A simple method to monitor serum concentrations of fluoxetine and its major metabolite for pharmacokinetic studies[J]. J Pharm Biomed Anal. 2009, 49(2):554.
[8]	肖红, 周群, 张石宁, 等. HPLC测定人血清中氟西汀的浓度[J]. 江苏药学与临床研究, 2000,8(4):36 .
[9]	李玲,袁波,朱荣申, 等. 高效液相色谱法检测抑郁症患者血清中氟西汀的浓度[J]. 中国药学杂志, 2002, 37(l1):855.
[10]	邵庆翔,李川,张美云,等. 盐酸氟西汀分散片和胶囊的生物等效性研究[J]. 中国新药杂志,2003,12(4):277.

Message Board

Determination of fluoxetine hydrochloride in plasma by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.019

Abstract

References

Proportional views

通讯作者: 陈斌, [email protected]

Article Metrics

Related

Proportional views