Determination of the sulfate content in sulfated fucans by barium sulfate turbidity

CHEN Qian; MA Tian-xiang; GUO Hong-ju; SHI Ning; WU Jiu-hong

doi:10.3969/j.issn.1006-0111.2012.02.011

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Objective To determine the sulfate content in sulfated fucans (FPS) by Barium sulfate turbidity. Methods With spectrophotometer at 360 nm, the sulfate content was determined by Barium Sulfate Turbidity. Results There was a good linearity (r²=0.990 9) when the sulfate content was within the range of 60~120 μg. Sulfate content of FPS was (20.52±0.57)% under the determination method.Methodology met the measurement requirement. Conclusion The method was proved to be reliable, quick, sensitive and applicable.

Determination of the sulfate content in sulfated fucans by barium sulfate turbidity

1. Anhui Medical University, Hefei 230000, China;306th Hospital of PLA, Beijing 100101, China

2. 306th Hospital of PLA, Beijing 100101, China

Received Date: 2011-09-15

Rev Recd Date: 2011-12-01

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Abstract: Objective To determine the sulfate content in sulfated fucans (FPS) by Barium sulfate turbidity. Methods With spectrophotometer at 360 nm, the sulfate content was determined by Barium Sulfate Turbidity. Results There was a good linearity (r²=0.990 9) when the sulfate content was within the range of 60~120 μg. Sulfate content of FPS was (20.52±0.57)% under the determination method.Methodology met the measurement requirement. Conclusion The method was proved to be reliable, quick, sensitive and applicable.

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[1]	Wang J, Zhang QB, Zhang ZS.et al.Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica[J]. Int J Biol Macromol,2010, 46(1):6.
[2]	陈乾,吴久鸿,郭宏举.褐藻糖胶对⁶⁰Co-γ射线照射引起的人外周血淋巴细胞微核率的影响[J].解放军药学学报,2011,27(5):383.
[3]	Lee J, Kim J, Moon C.et al.Radioprotective effects of fucoidan in mice treated with total body irradiation[J].Phytother Res,2008,22(12):1677.
[4]	Ponce NM, Pujol CA, Damonte EB. et al. Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies[J].Carbohydr Res,2003,338(2):153.
[5]	王君,胡霞敏.海藻提取物Fucoidan抗肿瘤作用的实验研究[J].时珍国医国药,2009, 20(7):1757.
[6]	樊文乐,武文洁.褐藻糖胶中硫酸根质量分数的测定方法[J].化学工业与工程技术,2005,26(5):47.
[7]	肖策,李多伟,逄敏洁.从海带中提取褐藻糖胶工艺的研究[J].西北药学杂志,2008,23(4):212.
[8]	余华.海带多糖中蛋白质去除方法的对比研究[J].成都大学学报:自然科学版,2005,24(4):265.
[9]	丛建波,王长振,李妍.褐藻硫酸多糖硫酸基含量测定-硫酸钡比浊法研究[J].解放军药学学报,2003,19(3):181.
[10]	宫春宇,潘迎捷.硫酸钡比浊法对龙须菜多糖中硫酸基含量的测定[J].天然产物研究与开发,2009,21(B10):402.
[11]	张维杰.糖复合物生化研究技术[M].第2版.杭州:浙江大学出版社,1999:91~92.

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Determination of the sulfate content in sulfated fucans by barium sulfate turbidity

doi: 10.3969/j.issn.1006-0111.2012.02.011

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通讯作者: 陈斌, [email protected]

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