WANG Xi-ying, ZHU Quan-gang, GAO Jing, GAO Shen, PAN Yong-hua, MIAO Peng. Protective effect of α-lipoic acid on noise induced hearing loss in guinea pigs[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(2): 103-106. doi: 10.3969/j.issn.1006-0111.2012.02.007
| Citation:
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WANG Xi-ying, ZHU Quan-gang, GAO Jing, GAO Shen, PAN Yong-hua, MIAO Peng. Protective effect of α-lipoic acid on noise induced hearing loss in guinea pigs[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(2): 103-106. doi: 10.3969/j.issn.1006-0111.2012.02.007
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Protective effect of α-lipoic acid on noise induced hearing loss in guinea pigs
- 1.
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
- 2.
School of pharmacy, Second Military Medical University, Shanghai 200433, China
- 3.
Bengbu Medical College, Bengbu 233030, China
- Received Date: 2011-11-24
- Rev Recd Date:
2012-01-11
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Abstract
Objective To observe the protection of free radical scavenger: α-lipoic acid on the explosive hearing loss in rats. Methods 24 pure white male guinea pigs weighted between 250~300 g, which were randomly divided into α-lipoic acid low, medium, high group(n=3×6), Noise controlled group(n=6). Each group of animals was exposed to octave band noise (4 kHz center frequency) 115 dB SPL 4 h. α-Lipoic acid group were administered α-lipoic acid 30 mg/(kg·d), 60 mg/(kg·d), 120 mg/(kg·d) for 10 days (2 days before the exposure, 7 days after); Noise controlled group animals were given normal saline orally. After exposure, auditory brainstem response (ABRs) was tested in animals in each group at different times; hair cell loss and damage were examined through histological means. Results After exposure of 3 days, lower/middle/ high dose group had significant differences with noise control group at frequencies 8, 16 kHz; low and high dose group had significant differences with noise control group at frequencies 24, 32 kHz; 7 days after exposure, middle/ high dose group had significant differences with noise control group at frequencies of 8, 16, 24 kHz), low and high dose group had significant differences with noise control group at frequencies 32 kHz; after 10 days, high dose group had significant differences with noise control group at each frequencies. In the histological examination, animals of three α-lipoic acid dose group were found little inside and outside hair cell loss or damage. Noise controlled animals showed outer hair cells in the ear edema/vacuolar degeneration and other diseases. Conclusions Low, middle, high doses of a-lipoic acid on the explosive hearing loss had a preventive effect. High dose α-lipoic acid could take a good prevention and treatment in noise-induced hearing loss.
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References
|
[1]
|
Seidman MD, Khan MJ, Bai U,et al. Biologic activity of mitochondrial metabolites on aging and age-related hearing loss[J]. Am J Otol, 2000, 21:161. |
|
[2]
|
郑贵亮,翟所强.噪声性耳聋的发病机制研究进展[J].山东医药,2008,48(15):115. |
|
[3]
|
Le Prell CG, Yamashita D, Minamic SB, et al. Mechanisms of noise-induced hearing loss indicate multiple methods of prevention[J]. Hear Res, 2007, 226:22. |
|
[4]
|
刁明芳,刘海瑛,张琰敏,等.噪声刺激后豚鼠耳蜗抗氧化能力的变化和α-硫辛酸对声损伤的保护作用[J].生理学报,2003,55(6):672. |
|
[5]
|
Subramaniam M, Salvi RJ, Spongr VP, et al. Changes in distortion product otoacoustic emissions and outer hair cells following interrupted noise exposures[J].Hear Res,1994,74:204. |
|
[6]
|
Wu HP,Hsu CJ,Cheng TJ, et al.N-acetylcysteine attenuates noise-induced permanent hearing loss in diabetic rats[J]. Hear Res, 2010, 267: 71. |
|
[7]
|
Coleman JKM, Kopke RD, Liu J, et al.Pharmacological rescue of noise induced hearing loss using N-acetylcysteine and acetyl-L-carnitine[J]. Hear Res,2007,226: 104. |
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