Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

CHEN Qian; CHEN Meng-li; YE Zhao-bo

doi:10.3969/j.issn.1006-0111.2012.01.006

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Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.

Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

1. Department of clinical pharmacology research, General Hospital of PLA, Beijing 100853 China

2. College of traditional Chinese medicine , The Chinese University of Hong Kong , Hong Kong,China

Received Date: 2011-08-11

Rev Recd Date: 2011-10-14

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Abstract: Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.

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Reference (13)

[1]	庄辉. ALD 的流行病学[J]. 中华肝脏病杂志, 2003, 11: 698.
[2]	全国酒精性肝病调查协作组. 全国酒精性肝病的多中心调查分析[J]. 中华消化杂志 2007; 27: 231.
[3]	中国药典[M]. 2005版1部. 2005: 364.
[4]	Chen ML, Tsai SH, Ip SP, et al. Long-term treatment with a "Yang-Invigorating" Chinese herbal formula, WuZi-YanZongWan, reduces mortality and liver oxidative damage in chronic alcohol-Intoxicated rats[J]. Rejuv Res, 2010, 13: 459.
[5]	Chen ML, Ip SP, Tsai SH, et al. Biochemical mechanism of Wu-Zi-Yan-Zong-Wan, a traditional Chinese herbal formula, against alcohol-induced oxidative damage in CYP2E1 cDNA-transfected HepG2 (E47) cells[J]. J Ethnopharmacol, 2010, 128: 116.
[6]	Ronis MJ, Hakkak R, Korourian S, et al. Alcoholic liver disease in rats fed ethanol as part of oral or intragastric low-carbohydrate liquid diets[J]. Exp Biol Med 2004; 229: 351.
[7]	Albano E. New concepts in the pathogenesis of alcoholic liver disease[J]. Expert Rev Gastroenterol Hepatol 2008; 2:749.
[8]	Arteel GE. Oxidants and antioxidants in chronic alcoholic liver disease[J]. Gastroenterology, 2003, 124: 778.
[9]	Hanck C, Rossol S, Bocker U, et al. Presence of plasma endotoxin is correlated with tumour necrosis factor receptor levels and disease activity in alcoholic cirrhosis[J]. Alcohol Alcoholism, 1998, 33:606.
[10]	Tilg H, Wilmer A, Vogel W, et al. Serum levels of cytokines in chronic liver diseases[J]. Gastroenterology, 1992, 103: 264.
[11]	Tilg H, Jalan R, Kaser A, et al. Anti-tumor necrosis factor-α monoclonal antibody therapy in severe alcoholic hepatitis[J]. J Hepatol, 2003, 38: 419.
[12]	Menon K, Stadheim L, Kamath P, et al. Safety and tolerability of etanercept in patients with alcoholic hepatitis[J]. Am J Gastroenterol, 2004, 99: 255.
[13]	Spahr L, Rubbia-Brandt L, Frossard J, et al. Combination of steroids with infliximab or placebo in severe alcoholic hepatitis: a randomized controlled pilot study[J]. J Hepatol, 2002, 37: 448.

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Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

doi: 10.3969/j.issn.1006-0111.2012.01.006

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通讯作者: 陈斌, [email protected]

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